Antiproliferative activity and induction of apoptotic by ethanolic extract of Alpinia galanga rhizhome in human breast carcinoma cell line

BMC Complementary and Alternative Medicine

Mohadeseh Hosseini²  Jalil Tavakkol Afshari⁴  Mousa-Al-Reza Hadjzadeh¹  Saeed Samarghandian³ 

[1] Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran;Department of Biology, Payame Nour University of Tehran, Tehran, Iran;Department of Basic Medical Sciences, Neyshabur University of Medical Sciences, Neyshabur, Iran;Immunogentics and Cell Culture Department, Immunology Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran

关键词: MTT;    MRC-5;    MCF-7;    Cytotoxicity;    Alpinia galanga L;   

Others  :  1220092
DOI  :  10.1186/1472-6882-14-192

 received in 2013-06-18, accepted in 2014-05-08,  发布年份 2014

【 摘 要 】

Background

We investigated the potential of galangal rhizomes to induce cytotoxic and apoptotic effects in the cultured human breast carcinoma cell line, (MCF-7) in compare with the non-malignant (MRC-5) cells.

Methods

Both cells were cultured in DMEM medium and treated with galangal rhizomes for three consecutive days. The percentage of apoptotic cells was determined by flow cytometry using Annexin-V fluorescein isothiocyanate.

Results

The results showed that the ethanolic extract of galangal rhizomes decreased cell viability in the malignant cells as a concentration- and time- dependent manner. The IC₅₀ values against MCF-7 were determined at 400.0 ± 11.7 and 170.0 ± 5.9 μg/ml after 48 and 72 h respectively. The morphology of MCF-7 cells treated with the ethanolic extract confirmed the cell proliferation assay results. Alpinia galanga induced apoptosis in MCF-7 cells, as determined by flow cytometry.

Conclusions

We concluded that the extract of Alpinia galanga exerts pro-apoptotic effects in a breast cancer-derived cell line and could be considered as a potential chemotherapeutic agent in breast cancer.

【 授权许可】

   
2014 Samarghandian et al.; licensee BioMed Central Ltd.

【 图 表 】

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