Evolution of MHC class I genes in the endangered loggerhead sea turtle (Caretta caretta) revealed by 454 amplicon sequencing

BMC Evolutionary Biology

Christophe Eizaguirre³  Frédéric J J Chain³  Sonia E Merino¹  Victor A Stiebens² 

[1] National Institute for Development and Fishery (INDP), Mindelo, Cape Verde;Department of Evolutionary Ecology of Marine Fishes, GEOMAR

[2] Helmholtz Center for Ocean Research, Kiel, 24105, Germany;Department of Evolutionary Ecology, Max Planck Institute for Evolutionary Biology, Ploen, 24306, Germany

关键词: Intermediate diversity;    Reptiles;    Trans-species polymorphism;    Loggerhead sea turtle;    Major histocompatibility complex;   

Others  :  1087452
DOI  :  10.1186/1471-2148-13-95

 received in 2013-01-06, accepted in 2013-04-17,  发布年份 2013

【 摘 要 】

Background

In evolutionary and conservation biology, parasitism is often highlighted as a major selective pressure. To fight against parasites and pathogens, genetic diversity of the immune genes of the major histocompatibility complex (MHC) are particularly important. However, the extensive degree of polymorphism observed in these genes makes it difficult to conduct thorough population screenings.

Methods

We utilized a genotyping protocol that uses 454 amplicon sequencing to characterize the MHC class I in the endangered loggerhead sea turtle (Caretta caretta) and to investigate their evolution at multiple relevant levels of organization.

Results

MHC class I genes revealed signatures of trans-species polymorphism across several reptile species. In the studied loggerhead turtle individuals, it results in the maintenance of two ancient allelic lineages. We also found that individuals carrying an intermediate number of MHC class I alleles are larger than those with either a low or high number of alleles.

Conclusions

Multiple modes of evolution seem to maintain MHC diversity in the loggerhead turtles, with relatively high polymorphism for an endangered species.

【 授权许可】

   
2013 Stiebens et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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