Putative interchromosomal rearrangements in the hexaploid wheat (Triticum aestivum L.) genotype ‘Chinese Spring’ revealed by gene locations on homoeologous chromosomes

BMC Evolutionary Biology

Chunji Liu¹  Jaroslav Doležel³  Guijun Yan¹  You-Liang Zheng²  Yuming Wei²  Zhi Zheng¹  Jiri Stiller⁴  Jian Ma⁴ 

[1] School of Plant Biology, The University of Western Australia, Perth 6009, WA, Australia;Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu 611130, China;Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany, Šlechtitelů 31, Olomouc, CZ-78371, Czech Republic;CSIRO Agriculture Flagship, 306 Carmody Road, St Lucia 4067, QLD, Australia

关键词: Chinese Spring;    Comparative genomics;    Translocation;    Wheat genome;    Interchromosomal rearrangements;   

Others  :  1158290
DOI  :  10.1186/s12862-015-0313-5

 received in 2014-11-05, accepted in 2015-02-20,  发布年份 2015

【 摘 要 】

Background

Chromosomal rearrangements are a major driving force in shaping genome during evolution. Previous studies show that translocated genes could undergo elevated rates of evolution and recombination frequencies around these genes can be altered. Based on the recently released genome sequences of Triticum urartu, Aegilops tauschii, Brachypodium distachyon and bread wheat, an analysis of interchromosomal translocations in the hexaploid wheat genotype ‘Chinese Spring’ (‘CS’) was conducted based on chromosome shotgun sequences from individual chromosome arms of this genotype.

Results

A total of 720 genes representing putative interchromosomal rearrangements was identified. They were distributed across the 42 chromosome arms. About 59% of these translocated genes were those involved in the well-characterized translocations involving chromosomes 4A, 5A and 7B. The other 41% of the genes represent a large numbers of putative interchromosomal rearrangements which have not yet been described. The number of the putative translocation events in the D subgenome was about half of those presented in either the A or B subgenomes, which agreed well with that the times of interaction between the A and B subgenomes almost doubled that between either of them and the D subgenome.

Conclusions

The possible existence of a large number of interchromosomal rearrangements detected in this study provide further evidence that caution should be taken when using synteny in ordering sequence contigs or in cloning genes in hexaploid wheat. The identification of these putative translocations in ‘CS’ also provide a base for a systematic evaluation of their presence or absence in the full spectrum of bread wheat and its close relatives, which could have significant implications in a wide array of fields ranging from studies of systematics and evolution to practical breeding.

【 授权许可】

   
2015 Ma et al.; licensee BioMed Central.

【 图 表 】

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