Nutritional evaluation, antioxidant studies and quantification of poly phenolics, in Roscoea purpurea tubers

BMC Research Notes

Ajay Kumar Singh Rawat¹  Shikhar Verma¹  Sharad Srivastava¹  Ankita Misra¹ 

[1] Pharmacognosy and Ethanopharmacology Division, CSIR-National Botanical Research Institute, Lucknow 226001, India

关键词: Poly phenolics;    Invitro assays;    HPTLC;    Nutritional characterization;    Roscoea purpurea;   

Others  :  1230913
DOI  :  10.1186/s13104-015-1290-x

 received in 2014-12-10, accepted in 2015-07-22,  发布年份 2015

【 摘 要 】

Background

Roscoea purpurea (Zingiberaceae) is commonly known as “kakoli”. Traditionally, various parts like leaves, roots and flower etc. are used for the treatment of diabetic, hypertension, diarrhea, fever, inflammation etc. In Nepal tubers are boiled for edible purpose and also used in traditional veterinary medicine. The study aims for nutritional characterization, chemical profiling of R. purpurea (tubers) methanol extract (RPE) along with evaluation of its anti-oxidant activity. Physicochemical and nutritional content were estimated as per standard protocols. Chemical profiling of markers includes method optimization, identification & quantification of bioactive poly phenolics through HPTLC. Anti oxidant potential RPE was analyzed via. Total phenolics (TPC), total flavonoids (TFC), reducing power assay, DPPH and β-carotene bleaching model.

Results

Physicochemical and nutritional standards were established. Kaempferol (0.30%), vanillic acid (0.27%), protocatechuic (0.14%), syringic (0.80%) and ferulic acid (0.05%) were identified and then quantified. TPC and TFC content were found to be 7.10 ± 0.115 and 6.10 ± 0.055%, reducing power of extract also increases linearly (r ²  = 0.946) with concentration, similar to standards. IC ₅₀value of extract in DPPH and β-carotene bleaching model was observed at 810.66 ± 1.154 and 600.66 ± 1.154 µg/ml, which is significantly different from standards (p < 0.05). Although there is a positive, significant correlation between the phenolic and flavonoid content with anti oxidant activity of extract.

Conclusion

Thus, study will authenticates the identity, utility of herb as nutrient supplement and an important medicinal plant having promising pharmacological activities for further elaborated/extended investigation work.

【 授权许可】

   
2015 Misra et al.

【 图 表 】

Fig.1.

Fig.2.

Fig.3.

Fig.4.

Fig.5.

【 参考文献 】

• [1]N Handa Flora of India. http://www.arvindguptatoys.com/arvindgupta/nimrethanda.pdf. Accessed 16 Oct 2014
• [2]Singh G, Rawat GS: Ethnomedicinal survey of Kedarnath wildlife sanctuary in Western Himalaya, India. Indian J Fundamen App Life Sci 2011, 1(1):35-36.
• [3]Singh AP: Ashtavarga—rare medicinal plants. Ethnobot Leaflets 2006, 10:104-108.
• [4]Sahu MS, Mali PY, Waikar SB, Rangari VD: Evaluation of immuno modulatory potential of ethanolic extract of Roscoea procera rhizomes in mice. J Pharm Bioallied Sci 2010, 2(4):346-349.
• [5]Bairwa R, Basyal D, Srivastav B: Study of antidiabetic and hypolipidemic activity of Roscoea purpurea (zingiberaceae). Int J Institut Pharm Life Sci 2012, 2(4):130-137.
• [6]Chinmay R, Kumari S, Dhar B, Mohanty RC, Dixit R, Padhi MM, et al.: Pharmacognostical and phytochemical studies of Roscea procera (Kakoli) and Lilium Polyphyllum (Ksheerkakoli) in comparison with market samples. Pharma J 2011, 3(25):32-38.
• [7]Anonymous: Indian pharmacopeia. Government of India: Ministry of health and Family welfare, New Delhi; 1996.
• [8]Anonymous: The ayurvedic pharmacopoeia of India. Government of India: Ministry of Health and Family Welfare, New Delhi; 1989.
• [9]Bray HC, Thorpe WV: Analysis of phenolic compounds of interest in metabolism. Meth Biochem Anal 1954, 1:27-52.
• [10]Ordon Ez AAL, Gomez JD, Vattuone MA, Isla MI: Antioxidant activities of Sechium edule (Jacq.) Swart extracts. Food Chem 2006, 97:452-458.
• [11]Harborne JB: Phytochemical methods. Chapman and Hall, London; 1973.
• [12]Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with folin phenol reagent. J Bio Chem 1951, 193:265-275.
• [13]Mukherjee PK: Quality control of herbal drugs formulation, an approach to evaluation of herbal drug formulations. Galaxy printers, Business Horizons, New Delhi; 2008.
• [14]ICH guidelines Q2R1, Validation of analytical procedures: text methodology, Geneva, Switzerland, November 1996/2005. http://www.ich.org. Accessed 16 Oct 2014
• [15]Kumaran A, Karunakaran RJ: In vitro antioxidant activities of methanol extract of Phyllanthus species from India. Lebens Wiss Techn 2007, 40:344-352.
• [16]Liyana-Pathiranan CM, Shahidi F: Antioxidant activity of commercial soft and hard wheat (Triticum aestivum) as affected by gastric pH conditions. Agric Food Chem 2005, 53:2433-2434.
• [17]Adegako GO, Vijay KM, Gopal KAG, Vardaraj MC, Sambaiah K, Lokesh BR: Antioxidants and lipid oxidation in foods. J Food Sci Tech 1998, 35:283-288.
• [18]Owolabi AO, Ndidi US, James BD, Amune FA: Proximate, anti nutrient and mineral composition of five varieties of coepea, Vigna unguiculata, commonly consumed in samara community, Zaria-Nigeria. Asian J Food Sci Tech 2012, 4(2):70-72.
• [19]Frankel EN, Huang SW, Kanner J, German JB: Interfacial phenomena in the evaluation of antioxidants: bulk oils versus emulsions. J Agri Food Chem 1994, 42:1054-1059.
• [20]Koleva II, Van Beek TA, Linssen JPH, De Groot A, Evstatieva LN: Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochem Anal 2002, 13:8-17.
• [21]Porter WL: Paradoxal behaviour of antioxidants in food and biological systems. In Antioxidants, chemical, physiological, nutritional and toxicological aspects. Edited by Wiliams GM. Princeton Scientific, Princeton; 1993.