Reelin expression in brain endothelial cells: an electron microscopy study

BMC Neuroscience

Hector J Caruncho²  Erin Y Fenton²  Emma Perez-Costas¹ 

[1] Department of Psychology, University of Alabama at Birmingham, College of Arts and Sciences, Campbell Hall 415, 1720 2nd Avenue South, Birmingham 35294, Alabama, USA;Division of Pharmacy, College of Pharmacy and Nutrition, University of Saskatchewan, 1B23 Health Sciences Building, 107 Wiggins Road, Saskatoon S7N 5E5, SK, Canada

关键词: Brain capillaries;    Transcytosis;    Blood-brain-barrier;   

Others  :  1170552
DOI  :  10.1186/s12868-015-0156-4

 received in 2014-11-04, accepted in 2015-03-11,  发布年份 2015

【 摘 要 】

Background

Reelin expression and function have been extensively studied in the brain, although its expression has been also reported in other tissues including blood. This raises the possibility that reelin might be able to cross the blood-brain barrier, which could be functionally relevant. Up-to-date no studies have been conducted to assess if reelin is present in the blood-brain barrier, which is mainly constituted by tightly packed endothelial cells. In this report we assessed the expression of reelin in brain capillaries using immunocytochemistry and electron microscopy.

Results

At the light microscope, reelin immunolabeling appeared in specific endothelial cells in brain areas that presented abundant diffuse labeling for this protein (e.g., layer I of the cortex, or the stratum lacunosum moleculare of the hippocampus), while it was mostly absent from capillaries in other brain areas (e.g., deeper cortical layers, or the CA1 layer of the hippocampus). As expected, at the electron microscope reelin labeling was observed in neurons of the cortex, where most of the labeling was associated with the rough endoplasmic reticulum. Importantly, reelin was also observed in some endothelial cells located in small capillaries, which confirmed the findings obtained at the light microscope. In these cells, reelin labeling was located primarily in caveolae (i.e., vesicles of transcytosis), and associated with the plasma membrane of the luminal side of endothelial cells. In addition, some scarce labeling was observed in the nuclear membrane.

Conclusions

The presence of reelin immunolabeling in brain endothelial cells, and particularly in caveolar vesicles within these cells, suggests that reelin and/or reelin peptides may be able to cross the blood-brain barrier, which could have important physiological, pathological, and therapeutic implications.

【 授权许可】

   
2015 Perez-Costas et al.; licensee BioMed Central.

【 图 表 】

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