Variation of chemosensory receptor content of Campylobacter jejuni strains and modulation of receptor gene expression under different in vivo and in vitro growth conditions

BMC Microbiology

Victoria Korolik²  Evgeny A Semchenko²  Serena K Day¹  Greg Tram²  Rebecca M King²  Lucy K Shewell²  Lauren E Hartley-Tassell²  Christopher J Day² 

[1] Department of Microbiology, Pathology Queensland, Gold Coast Hospital, Southport, Australia;Institute for Glycomics, Griffith University Gold Coast Campus, Griffith University, Griffith, Australia

关键词: Transducer-like proteins;    Campylobacter;    Chemotaxis receptor;   

Others  :  1221843
DOI  :  10.1186/1471-2180-12-128

 received in 2012-03-21, accepted in 2012-06-29,  发布年份 2012

【 摘 要 】

Background

Chemotaxis is crucial for the colonisation/infection of hosts with Campylobacter jejuni. Central to chemotaxis are the group A chemotaxis genes that are responsible for sensing the external environment. The distribution of group A chemoreceptor genes, as found in the C. jejuni sequenced strains, tlp1-4, 7, 10 and 11 were determined in 33 clinical human and avian isolates.

Results

Group A tlp gene content varied among the strains with genes encoding tlp1 (aspartate receptor, ccaA) and tlp7 present in all strains tested, where as tlp11 was present in only one of our international collection clinical isolates, C. jejuni 520, but was more prevalent (9/13) in the freshly isolated clinical stains from patients who required hospitalisation due to C. jejuni infection (GCH1-17). Relative expression levels of the group A tlp genes were also determined in C. jejuni reference strains NCTC 11168-GS, 11168-O and 81116 using cells grown in vitro at 37°C, 42°C and maintained at room temperature and with cells isolated directly from murine and avian hosts by immune magnetic separation without subsequent culture. Gene expression of tlp genes was varied based on strain, growth conditions and in vivo isolation source. Tlp1, although the most conserved, showed the lowest and most varied mRNA expression and protein production under laboratory conditions. Tlp7 was highly expressed at most conditions tested, and gene expression was not influenced by the tlp7 gene encoding a full length protein or one expressed as separate periplasmic and cytoplasmic domains.

Conclusion

We have shown that chemosensory receptor set variation exists among C. jejuni strains, but is not dependent on the isolation source.

【 授权许可】

   
2012 Day et al.; licensee BioMed Central Ltd.

【 图 表 】

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