Medicinal attributes of Solanum xanthocarpum fruit consumed by several tribal communities as food: an in vitro antioxidant, anticancer and anti HIV perspective

BMC Complementary and Alternative Medicine

Abhay K Pandey¹  Shashank Kumar¹ 

[1] Department of Biochemistry, University of Allahabad, Allahabad 211002, India

关键词: Anti HIV;    Solanum xanthocarpum;    Reducing power;    LPOI;    DPPH;    Anticancer;    Antioxidant;   

Others  :  1220211
DOI  :  10.1186/1472-6882-14-112

 received in 2013-12-18, accepted in 2014-03-24,  发布年份 2014

【 摘 要 】

Background

Solanum xanthocarpum (Solanaceae) has been used for treatment of many infectious and degenerative diseases in traditional medicine. Present study reports the medicinal efficacy of S. xanthocarpum fruit as antioxidant, anticancer and anti HIV agents.

Methods

Extracts were prepared using Soxhlet apparatus and partially characterized by thin layer chromatography (TLC). Total flavonoid content was determined spectrophotometrically. Reducing power, DPPH radical scavenging activity and lipid peroxidation inhibition assays were used for measurement of antioxidant potential. Cytotoxic (SRB assay) and anti-HIV RT inhibition (RT assay kit, Roche) activities were determined using ELISA.

Results

TLC revealed the diversity of phytoconstituents in various sequential extracts of S. xanthocarpum fruit. Total flavonoid contents in extracts ranged between 10.22–162.49 μg quercetin equivalent/mg. Spectroscopic scanning of water soluble phenolics showed maximum absorbance at 250 and 280 nm. Polar extracts displayed potent radical scavenging activity (>80%). Several sub-fractions (spots) of extracts separated on TLC plates also exhibited powerful radical scavenging activity. Considerable reducing power was observed in extracts. Hexane fraction provided 55% lipoprotection in rat kidney homogenate. Non-polar extracts exhibited appreciable cytotoxic activity (70-91%) against leukemia (THP-1) and lung cancer (HOP-62) cell lines. Lower inhibitory activity was observed in extracts against HIV Reverse Transcriptase enzyme.

Conclusion

The study demonstrated considerable antioxidant and anticancer activities in S. xanthocarpum fruit.

【 授权许可】

   
2014 Kumar and Pandey; licensee BioMed Central Ltd.

【 图 表 】

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