Qiwei granules alleviates podocyte lesion in kidney of diabetic KK-Ay mice

BMC Complementary and Alternative Medicine

Tonghua Liu³  Ming Gao²  Tunhai Xu³  Xinli Wu³  Yan Zhang³  Lili Wu³  Xiangyu Guo³  Lingling Qin³  Linyi Li³  Hisae Yoshitomi²  Wen Sun³  Jingxin Zhou¹ 

[1] Dongzhimen Hospital Eastern Affilated to Beijing University of Chinese Medicine, 116 Cuiping West Road, Tongzhou District, Beijing, 100029, People’s Republic of China;School of Pharmaceutical Sciences, Mukogawa Women’s University, 11-68 Koshien Kyuban-cho, Nishinomiya 663-8179, Hyogo, Japan;Beijing University of Chinese Medicine, 11 North 3rd-ring East Road, Chaoyang District, Beijing, 100029, People’s Republic of China

关键词: Akt signaling pathway;    Slit diaphragm;    Podocyte;    KK-Ay mice;    Diabetic nephropathy;    Qiwei granules;   

Others  :  1219792
DOI  :  10.1186/s12906-015-0603-x

 received in 2014-07-22, accepted in 2015-03-09,  发布年份 2015

【 摘 要 】

Background

Chinese medicine comprised of all natural herbs is widespread used in the treatment of diabetic nephropathy (DN). Podocyte contributes to the integrity of glomerular filtration barrier whose injury plays an important role in the initiation and progression of DN. Our study aimed to investigate the effect of Qiwei granules on podocyte lesion in diabetic KK-A^y mice kidney and its underlying mechanism.

Methods

Twelve-week-old male KK-A^y mice were randomly divided in vehicle group and Qiwei granules group, while C57BL/6J mice were used as normal control. The mice were gavage with 1.37 g/kg/day Qiwei granules or water for 10 weeks. We measured water, food intake and body weight (BW) and fasting blood glucose (FBG) every 2 weeks, and urine protein every 4 weeks. At the end of the experiment, all surviving mice were sacrificed. The kidney weight and serum renal parameters were measured, and the renal morphology was observed. To search the underlying mechanism, we examined the podocyte positive marker, slit diaphragm protein expression and some involved cell signal pathway.

Results

Qiwei granules treatment significantly improved the metabolic parameters, alleviated the urinary protein, and protected renal function in KK-A^y mice. In addition, the glomerular injuries and podocyte lesions were mitigated with Qiwei granules treatment. Furthermore, Qiwei granules increased expression of nephrin, CD2AP, and integrin alpha3beta1 in the podocytes of KK-A^y mice. Qiwei granules improved the phosphoration of Akt and inhibited cleaved caspase-3 protein expression.

Conclusion

These finding suggest that Qiwei granules protects the podocyte from the development of DN via improving slit diaphragm (SD) molecules expression and likely activating Akt signaling pathway in KK-A^y mice.

【 授权许可】

   
2015 Zhou et al.; licensee BioMed Central.

【 图 表 】

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