A test of somatic mosaicism in the androgen receptor gene of Canada lynx (Lynx canadensis)

BMC Genetics

Paul J. Wilson²  Jeff Bowman³  Melanie B. Prentice¹ 

[1] Department of Environmental & Life Sciences, Trent University, 1600 West Bank Drive, Peterborough K9J 7B8, ON, Canada;Biology Department, Trent University, 1600 West Bank Drive, Peterborough K9J 7B8, ON, Canada;Wildlife Research and Monitoring Section, Ontario Ministry of Natural Resources and Forestry, 2140 East Bank Drive, Peterborough K9J 7B8, ON, Canada

关键词: Trinucleotide repeats;    Canada lynx;    Androgen receptor;    Somatic mosaicism;   

Others  :  1229694
DOI  :  10.1186/s12863-015-0284-y

 received in 2015-08-12, accepted in 2015-10-19,  发布年份 2015

【 摘 要 】

Background

The androgen receptor, an X-linked gene, has been widely studied in human populations because it contains highly polymorphic trinucleotide repeat motifs that have been associated with a number of adverse human health and behavioral effects. A previous study on the androgen receptor gene in carnivores reported somatic mosaicism in the tissues of a number of species including Eurasian lynx (Lynx lynx). We investigated this claim in a closely related species, Canada lynx (Lynx canadensis). The presence of somatic mosaicism in lynx tissues could have implications for the future study of exonic trinucleotide repeats in landscape genomic studies, in which the accurate reporting of genotypes would be highly problematic.

Methods

To determine whether mosaicism occurs in Canada lynx, two lynx individuals were sampled for a variety of tissue types (lynx 1) and tissue locations (lynx 1 and 2), and 1,672 individuals of known sex were genotyped to further rule out mosaicism.

Results

We found no evidence of mosaicism in tissues from the two necropsied individuals, or any of our genotyped samples.

Conclusions

Our results indicate that mosaicism does not manifest in Canada lynx. Therefore, the use of hide samples for further work involving trinucleotide repeat polymorphisms in Canada lynx is warranted.

【 授权许可】

   
2015 Prentice et al.

【 图 表 】

Fig. 1.

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