BMC Microbiology | |
Detection limits of Legionella pneumophila in environmental samples after co-culture with Acanthamoeba polyphaga | |
Valeria Gaia1  Simona Casati1  Lisa Conza1  | |
[1]Swiss National Reference Centre for Legionella, Cantonal Institute of Microbiology, Via Mirasole 22a, 6500, Bellinzona, Switzerland | |
关键词: Detection limit; Bioaerosol; Air; Compost; Co-culture; Culture; Legionella; | |
Others : 1144304 DOI : 10.1186/1471-2180-13-49 |
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received in 2012-11-21, accepted in 2013-02-08, 发布年份 2013 | |
【 摘 要 】
Background
The efficiency of recovery and the detection limit of Legionella after co-culture with Acanthamoeba polyphaga are not known and so far no investigations have been carried out to determine the efficiency of the recovery of Legionella spp. by co-culture and compare it with that of conventional culturing methods. This study aimed to assess the detection limits of co-culture compared to culture for Legionella pneumophila in compost and air samples. Compost and air samples were spiked with known concentrations of L. pneumophila. Direct culturing and co-culture with amoebae were used in parallel to isolate L. pneumophila and recovery standard curves for both methods were produced for each sample.
Results
The co-culture proved to be more sensitive than the reference method, detecting 102-103 L. pneumophila cells in 1 g of spiked compost or 1 m3 of spiked air, as compared to 105-106 cells in 1 g of spiked compost and 1 m3 of spiked air.
Conclusions
Co-culture with amoebae is a useful, sensitive and reliable technique to enrich L. pneumophila in environmental samples that contain only low amounts of bacterial cells.
【 授权许可】
2013 Conza et al; licensee BioMed Central Ltd.
【 预 览 】
Files | Size | Format | View |
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20150330113418771.pdf | 245KB | download | |
Figure 2. | 41KB | Image | download |
Figure 1. | 40KB | Image | download |
【 图 表 】
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Figure 2.
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