Co-operative inhibitory effects of hydrogen peroxide and iodine against bacterial and yeast species

BMC Research Notes

Mikhajlo K Zubko¹  Elena I Zubko¹ 

[1] School of Healthcare Science, Manchester Metropolitan University, John Dalton Building, Chester St, Manchester M1 5GD, UK

关键词: Genotoxicity;    Candida;    Saccharomyces;    Staphylococcus;    Pseudomonas;    Escherichia;    Hydrogen peroxide;    Iodine;    Synergism;    Antimicrobials;   

Others  :  1142158
DOI  :  10.1186/1756-0500-6-272

 received in 2013-02-27, accepted in 2013-07-04,  发布年份 2013

【 摘 要 】

Background

Hydrogen peroxide and iodine are powerful antimicrobials widely used as antiseptics and disinfectants. Their antimicrobial properties are known to be enhanced by combining them with other compounds. We studied co-operative inhibitory activities (synergism, additive effects and modes of growth inhibition) of hydrogen peroxide and iodine used concurrently against 3 bacterial and 16 yeast species.

Results

Synergistic or additive inhibitory effects were shown for hydrogen peroxide and iodine mixtures against all 19 species used in the study. Both biocides were mostly cidal individually and in mixtures against Pseudomonas aeruginosa and Staphylococcus aureus. Both compounds manifested static inhibitory effects individually, but their mixtures were synergistically cidal for Saccharomyces cerevisiae and Escherihia coli. Cells of S. cerevisiae treated with hydrogen peroxide and iodine-hydrogen peroxide mixture produced increased numbers of respiratory deficient mutants indicating genotoxic effects.

Conclusion

Iodine and hydrogen peroxide used concurrently interact synergistically or additively against a range of prokaryotic and eukaryotic microorganisms. The study provides an insight as to how these traditional antimicrobials could be used more effectively for disinfection and antisepsis. In addition, a simple approach is proposed for scoring genotoxicity of different biocides by using the budding yeast system.

【 授权许可】

   
2013 Zubko and Zubko; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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