In vitro antioxidant and antiproliferative activities of plants of the ethnopharmacopeia from northwest of Mexico

BMC Complementary and Alternative Medicine

Ramón Enrique Robles-Zepeda¹  Armando Burgos-Hernández²  Carolina Ortiz-Sandoval¹  Ricardo Lapizco-Vázquez¹  Davisela Sierras-Canchola¹  Adriana Garibay-Escobar¹  Carlos Velázquez-Contreras¹  Manuel Jiménez-Estrada³ 

[1] Departamento de Ciencias Químico Biológicas, División de Ciencias Biológicas y de la Salud, Universidad de Sonora, Blvd. Luis Encinas y Rosales Hermosillo, Sonora, 83000, México;Departamento de Investigación y Posgrado en Alimentos, División de Ciencias Biológicas y de la Salud, Universidad de Sonora, Encinas y Rosales Hermosillo, Sonora, 83000, México;Departamento de Productos Naturales, Instituto de Química, Universidad Nacional Autónoma de México, Cd. Universitaria, México, Distrito Federal, 04510, México

关键词: Medicinal plants Mexican;    Methanolic plants extracts;    Flavonoids;    Total phenol content;    DPPH;   

Others  :  1231164
DOI  :  10.1186/1472-6882-13-12

 received in 2012-09-20, accepted in 2013-01-04,  发布年份 2013

【 摘 要 】

Background

The aim of this study, is to investigate the in vitro antioxidant activity, the total phenols content, the flavonoids content and the antiproliferative activity of methanolic extracts of the plants: Krameria erecta, Struthanthus palmeri, Phoradendron californicum, Senna covesii and Stegnosperma halimifolium, used by different ethnic groups from northwestern Mexico in the treatment and cure of various diseases.

Methods

The in vitro antioxidant activity was measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and Ferric Reducing/Antioxidant Power assay (FRAP), the total phenols content was measured by Folin–Ciocalteau assay, the flavonoids content by the AlCl₃ colorimetric method and the antiproliferative activity (line cells HeLa, RAW 264.7, M12A^k.C3.F6 and L929) using MTT method.

Results

The K. erecta extract showed the higher radical scavenging activity (67.88%), antioxidant activity by FRAP (1.41 mg Trolox Eq), the highest total phenols content (598.51 mg Galic Acid Eq/g extract), the highest flavonoids content (3.80 mg Quercetin Eq/g extract) and the greatest antiproliferative activity in a dose dependent manner against most Cell line evaluated. A positive correlation was found between the antioxidant activity and the flavonoids content.

Conclusions

This study is the first report on the antioxidant and antiproliferative activities of the five species evaluated. The results demostrate that there is a positive correlation between antioxidant activity and the flavonoids content, indicating that these type of polyphenols could be the major contributors to the observed antioxidant activity in the evaluated plant extracts. Of the extracts evaluated, that of Krameria erecta showed the greatest antioxidant and antiproliferative activities, a discovery that makes this species a promising candidate for future research.

【 授权许可】

   
2013 Jiménez-Estrada et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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