MALDI-TOF mass spectrometry and identification of new bacteria species in air samples from Makkah, Saudi Arabia

BMC Research Notes

Didier Raoult⁶  Philippe Gautret¹  Steve Harakeh⁶  Ahmed M Hassan⁶  Ahmad M Ashshi²  Ziad A Memish³  Sarah Metidji⁴  Asad S Aburizaiza⁵  Fehmida Bibi⁶  Anastasia Papadioti⁴  Esam I Azhar⁷  Muhammad Yasir⁶  Emmanouil Angelakis⁴ 

[1]Infectious Diseases and Tropical Medicine Unit - North Hospital, Chemin des Bourrelys - 13915, Marseille, France

[2]Department of Laboratory Medicine, Faculty of Applied Medical Science, Umm Al-Qura University, Makkah, Saudi Arabia

[3]Ministry of Health, Riyadh, Saudi Arabia

[4]URMITE CNRS-IRD 198 UMR 6236, Université de la Méditerranée, Faculté de Médecine, 27 Bd Jean Moulin, 13385 Marseille, France

[5]Enviromental Science Department, Faculty of Metrology, Environmental Science and Arid Land Agriculture, King Abdulaziz University, Jeddah, Saudi Arabia

[6]Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia

[7]Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia

关键词: Saudi Arabia;    Air contamination;    New bacteria species;    Hajj season;    MALDI-TOF mass spectrometry;   

Others  :  1092374
DOI  :  10.1186/1756-0500-7-892

 received in 2014-05-05, accepted in 2014-11-25,  发布年份 2014

【 摘 要 】

Background

During the Hajj season, respiratory symptoms are very common among pilgrims. Here, we investigated the viable bacterial population in air samples collected around the slaughterhouses used during the Hajj.

Methods and results

We collected air samples on three days from four different sites: slaughterhouses at Al-Kakia, Al-Meaisim and Al-Sharaia, and from a waste disposal area designated for the remnants of slaughter. Samples were cultured on blood agar plates for 48 h, and bacterial isolates were identified using MALDI-TOF MS. A dendrogram using the spectra of the unidentified bacterial species was constructed, and PCR amplification and sequencing of the 16S rRNA gene was performed for one isolate per cluster. In total, 2500 colonies appeared on the nutrient agar plates, and 244 were purified for further analysis. Good identification was obtained for 202 (83%) isolates by MALDI-TOF MS. The most common genera were Bacillus (n = 94, 45%) and Staphyloccocus (n = 55, 26%). Poor identification was obtained for 42 (17%) isolates, and their spectra clustering revealed that these isolates belonged to 10 species. Four of these were considered to be new species.

Conclusions

During the Hajj, the air was contaminated by many environmental bacterial agents, and MALDI-TOF MS was successfully adapted for their rapid identification.

【 授权许可】

   
2014 Angelakis et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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