Laboratory assessment of molluscicidal and cercariacidal effects of Glinus lotoides fruits

BMC Research Notes

Yalemtsehay Mekonnen³  Mirutse Giday²  Berhanu Erko²  Gebrehiwot Kiros¹ 

[1] Wollo University, P.O. Box 1145, Dessie, Ethiopia;Aklilu Lemma Institute of Pathobiology, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia;College of Natural and Computational Sciences, Microbial, Cellular and Molecular Biology Department, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia

关键词: Ethiopia;    Schistosomiasis;    Cercariacide;    Molluscicide;    Glinus lotoides;   

Others  :  1133964
DOI  :  10.1186/1756-0500-7-220

 received in 2013-08-12, accepted in 2014-04-03,  发布年份 2014

【 摘 要 】

Background

The negative impact of synthetic molluscicides on the environment and their high cost necessitated search for an alternative approach of using plant extracts for the control of schistosomiasis. The objective of this study was, therefore, to evaluate aqueous and ethyl acetate crude extracts of Glinus lotoides fruits for their cercariacidal activity and molluscicidal effect against schistosome snail intermediate hosts.

Methods

Assessment of the molluscicidal activity against Biomphalaria pfeifferi was made by immersion method in accordance with WHO guideline. The results of mortality were statistically analyzed using probit analysis. The attenuating effect of the plant on Schistosoma mansoni cercariae was determined using establishment of adult worms as a parasitological parameter post exposure.

Results

The 24 and 48 hour-LC₅₀ values for the aqueous extract of G. lotoides fruits were 47.1 and 44.1 mg/L, respectively, whereas that of ethyl acetate were 66.1 and 59.6 mg/L, respectively. The 24 and 48 hour LC₉₀ values for the aqueous extract of G. lotoides fruits were 56.96 and 51.0 mg/L, respectively, while that of ethyl acetate were 77.2 and 70.0 mg/L, respectively. The in vitro cercariacidal activity was determined after 2 hrs of exposure to the aqueous plant extract. It was found out that the LC₅₀ and LC₉₀ values were 18.7 and 41.7 mg/L, respectively. Besides, infectivity of Schistosoma mansoni cercariae to mice was determined by exposing mice to cercariae pre-treated with the sub-lethal concentrations (3.7, 11.6 and 18.7 mg/L) of the aqueous extract. A significant reduction in worm burden in mice was obtained at 11.6 mg/L (p < 0.05). Moreover, the reduction in number of worms recovered was highly significant at 18.7 mg/L (p < 0.001).

Conclusions

The results showed that G. lotoides has molluscicidal activity against B. pfeifferi snails and cercariacidal activity against S. mansoni. Yet, further comprehensive evaluation is recommended for the possible use of G. lotoides against B. pfeifferi and the schistosome parasite.

【 授权许可】

   
2014 Kiros et al.; licensee BioMed Central Ltd.

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