Experimental studies on the effect of (Lambda-Cyhalothrin) insecticide on lungs and the ameliorating effect of plant extracts (Ginseng (Panax Ginseng) and garlic (Allium sativum L.) on asthma development in albino rats

BMC Research Notes

Aml Abd-Elkader¹  Amna M Mostafa¹  Mouchira M Mohi El-Din² 

[1] Zoo Department, Science Faculty, South Valley University, 83523 Qena, Egypt;Pathology and Clinical Pathology Department, Faculty of Veterinary Medicine, South Valley University, 83523 Qena, Egypt

关键词: Pathology;    Ginseng plants;    Garlic;    LTC;    Asthma;    Airway inflammation;    Albino rats;   

Others  :  1133434
DOI  :  10.1186/1756-0500-7-243

 received in 2013-09-05, accepted in 2014-04-11,  发布年份 2014

【 摘 要 】

Background

Lambda-cyhalothrin (LTC) is a synthetic pyrethroid insecticide for agricultural and public health applications. This study was to determine the pathological alterations of LTC in lungs, which has not previously been studied, and the ameliorating effects of plant extracts (ginseng and garlic) on the development of asthma in albino rats.

Methods

Four groups (gps) of albino rats, (n = 20, average body weight = 200 gm with an age of 4 months), were formed. Gp 1 was kept as control. Gp 2 was injected intraperitoneally (i.p.) with LTC at a dose of 1/6 LD50 that is 9.34 mg/kg body weight (w.t.) daily for 21 days (d). Gp 3 & 4 were injected (i.p.) with ginseng at the dose of 200 mg/kg b.wt and garlic (Allium sativum L.) at the dose of 100 mg/kg b.wt., respectively, one hour before being given LTC at a dose of 1/6 LD50 (9.34 mg/kg b.wt.) daily. Each groups were divided into two sacrificed, at 15 and 21 d p.i. Blood and lung samples were collected for hematological and histopathological examinations.

Results

Hematological findings showed that the animals in gps 2 and 3, which were treated for 21 days, showed a significant difference in RBC counts (P > .001), Hb (P > .007), PCV% (P > .004), (P > .008) in comparison with the control group. Signs of cough and nasal discharge were seen in gp 2, which became mild in gp 4. Grossly, the lungs showed congestion and consolidation in gp 2. Histopathologically, macroabscesses and interstitial alveolitis were seen in gp 2, which led to obstruction in the lumen of the bronchioles at 21 d p.i. Meanwhile, thickening in the interalveolar septa with mononuclear cells was seen in gps. 3 and 4 at 21d p.i.

Conclusions

The study shows 3 gps of rats injected with LHC alone or combined with garlic and ginseng extract, each group were divided into two sacrificed (15 and 21 d p.i.). Lambda cyhalothrin causes bronchial obstruction in the lungs of the rats (15 and 21 d p.i), which decreased into mild to moderate interstitial inflammation in the rats given garlic and ginseng, respectively.

【 授权许可】

   
2014 Mohi El-Din et al.; licensee BioMed Central Ltd.

【 图 表 】

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【 参考文献 】

• [1]Muhammad F, Akhtar M, Rahman Z u, Javed I, Anwar MI: Role of veterinarians in providing residue free animal food. Pak Vet J 2009, 29:42-46.
• [2]Baines KJ, Simpson JL, Bowden NA, Scott RJ, Gibson PG: Differential gene expression and cytokine production from neutrophils in asthma phenotypes. Eur Respir J 2010, 35:522-531.
• [3]Reigart JR, Roberts JR, Environmental Protection Agency (EPA): Recognition and Management of Pesticide Poisonings. Fifth edition. 1999. EPA 735-R-98-003[http://www.epa.gov/oppfead1/safety/healthcare/handbook/handbook.htm webcite]
• [4]Etzel RA: Pesticides and You Beyond Pesticides/National Coalition Against the Misuse of Pesticides (Asthma, Children and Pesticides, What you should know to protect your family). Vulnerability 701 E Street SE, Suite 200 Washington, DC 20003, 202-543-5450 2005, 25(2):18-21.
• [5]Mattei R, Dias RF, Espínola EB, Carlini EA, Barros SBM: Guarana (Paullinia cupana): toxic behavioral effects in laboratory animals and antioxidant activity in vitro. J Ethnopharmacol 1998, 60:111-116.
• [6]Wenzel SE, Szefler SJ, Leung DYM, Sloan SI, Rex MD, Martin RJ: Bronchoscopic evaluation of severe asthma: persistent inflammation associated with high dose glucocorticoids. Am J Respir Crit Care Med 1997, 156:737-743.
• [7]Augusti KT: Therapeutic values of onion (Allium cepa L.) and garlic (Allium sativum L.). Indian J Exp Biol 1996, 34:634-640.
• [8]Pesticide Fact Sheet Number 171: KARATE (PP321): U.S. Environmental Protection Agency, Office of Pesticide Programs. Washington, DC: U.S. Government Printing Office; 1988. npic.orst.edu/factsheets/l_cyhalotech.pdf
• [9]Alnaqeeb MA, Thomson M, Bordia T, Ali M: Histopathological effects of garlic on liver and lung of rats. Toxicol Lett 1996, 85:157-164.
• [10]Karadeniz A, Yildirim A, Çelebi F: Protective effect of panax ginseng against carbon tetrachloride (CCl4)- induced oxidative brain injury in rats. Atatürk Üni Vet Bil Derg 2007, 2(3):117-121.
• [11]Hoff J: Methods of Blood Collection in the Mouse. Lab Animal 2000, 29(10):47-53.
• [12]Borenstein M, Rothstein H, Cohen J: Sample power: Release 1.00. Chicago, IL: Statistical Package for the Social Sciences (SPSS); 1997.
• [13]Demian SR: Immunological alteration in mice exposed to deltamethrin insecticide: II. Defective humoral immune reactivity. J Med Res Inst 1998, 19(2):154-164.
• [14]David K, Traci P: Panax Ginseng. Am Fam Physician 2003, 68(8):1539-1542. Health & Medical Complete
• [15]Scaglione F, Ferrara F, Dugnani S, Falchi M, Santoro G, Fraschini F: Immunomodulatory effects of two extracts of Panax ginseng C.A. Meyer. Drugs Exp Clin Res 1990, 16:537-542.
• [16]Vogler BK, Pittler MH, Ernst E: The efficacy of ginseng. A systematic review of randomised clinical trials. Eur J Clin Pharmacol 1999, 55:567-575.
• [17]Mahady GB, Gyllenhall C, Fong HH, Farnsworth NR: Ginsengs: a review of safety and efficacy. Nutr Clin Care 2000, 3:90-101.
• [18]Lamm DL, Riggs DR: Enhanced immunocompetence by garlic: role in bladder cancer and other malignancies. J Nutr 2001, 131(3s):1067S-1070S.
• [19]Breuer R, Christensen TG, Lucey EC, Stone PJ, Snider GL: An ultrastructural morphometric analysis of elastase-treated hamster bronchi shows discharge followed by progressive accumulation of secretory granules. Am Rev Respir Dis 1987, 136:698-703.
• [20]Stanescu D, Sanna A, Veritier C, Kostianev S, Calcagni PG, Fabbri LM, Maestrelli P: Airways obstruction, chronic expectoration, and rapid decline of FEV1 in smokers are associated with increased levels of sputum neutrophils. Thorax 1996, 51:267-271.
• [21]Jatakanon A, Uasuf C, Maziak W, Lim S, Chung KF, Barnes PJ: Neutrophilic inflammation in severe persistent asthma. Am J Respir Crit Care Med 1999, 160:1532-1539.
• [22]Little SA, MacLeod KJ, Chalmers GW, Love JG, McSharry C, Thomson NC: Association of forced expiratory volume with disease duration and sputum neutrophils in chronic asthma. Am J Med 2002, 112:446-452.
• [23]Welsh MJ, Tsui LC, Boat TF, Beaudet AL: Cystic fibrosis. In The Metabolic and Molecular Bases of Inherited Diseases. Edited by Scriver CR, Beaudet AL, Sly WS, Valle D. New York: McGraw-Hill; 1995:3799-3876.
• [24]Senior RM, Shapiro SD: Chronic obstructive pulmonary disease: Epidemiology, pathophysiology, and pathogenesis. In Fishman’s Pulmonary Diseases and Disorders. Edited by Fishman AP. New York: McGraw-Hill; 1998:659-681.
• [25]Berry M, Morgan A, Shaw DE, Parker D, Green R, Brightling C, Bradding P, Wardlaw AJ, Pavord ID: Pathological features and inhaled corticosteroid response of eosinophilic and non-eosinophilic asthma. Thorax 2007, 62:1043-1049.
• [26]Jung ID, Kim HY, Park JW, Lee CM, Noh KT, Kang HK, Heo DR, Lee SJ, Son KH, Park HJ, Shin SJ, Park JH, Ryu SW, Seo JK, Park YM: RG-II from Panax ginseng C.A. Meyer suppresses asthmatic reaction. BMB Rep 2012, 45(2):79-84. doi: 10.5483
• [27]Kim DY, Yang WM: Panax ginseng ameliorates airway inflammation in an ovalbumin-sensitized mouse allergic asthma model. J Ethnopharmacol 2011, 14(1):230-235. doi:10.1016/j.jep.2011.04.048
• [28]Scaglione F, Weiser K, Alessandria M: Effects of the standardized ginseng extract G115® in patients with chronic bronchitis: a nonblinded, randomised, comparative pilot study. Clin Drug Invest [New Zealand] 2001, 21:41-45.
• [29]Best Foods for Preventing and Alleviating Asthma Symptoms-HealWithFood.org's Guide to Fighting Asthma (Food) (#9: Garlic), Is Garlic Good for Asthma Sufferers? Copyright ©2014. [http://www.healwithfood.org/asthma/foods.php#ixzz2ylZSeXPB webcite]
• [30]Yang Y, Wu T, He K, Fu ZG: Effect of aerobic exercise and ginsenosides on lipid metabolism in diet-induced hyperlipidemia mice. Acta Pharmacol Sin 1999, 20:563-565.