Single-cell phenomics reveals intra-species variation of phenotypic noise in yeast

BMC Systems Biology

Yoshikazu Ohya³  Joseph Schacherer²  Steffen Fehrmann¹  Yasutaka Imanaga³  Satoru Nogami³  Shinsuke Ohnuki³  Gaël Yvert¹ 

[1] Laboratoire de Biologie Moléculaire de la Cellule, Ecole Normale Supérieure de Lyon; CNRS, Université Lyon 1, 46 Allée d’Italie, Lyon F-69007, France;Department of Genetics, Genomics and Microbiology, University of Strasbourg; CNRS, Strasbourg, France;Department of Integrated Biosciences; Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Chiba, Japan

关键词: Bet hedging;    Complex traits;    Noise;    Stochasticity;    Cell morphology;    Yeast;    S. cerevisiae;    Single-cell;   

Others  :  1142721
DOI  :  10.1186/1752-0509-7-54

 received in 2012-10-04, accepted in 2013-06-21,  发布年份 2013

【 摘 要 】

Background

Most quantitative measures of phenotypic traits represent macroscopic contributions of large numbers of cells. Yet, cells of a tissue do not behave similarly, and molecular studies on several organisms have shown that regulations can be highly stochastic, sometimes generating diversified cellular phenotypes within tissues. Phenotypic noise, defined here as trait variability among isogenic cells of the same type and sharing a common environment, has therefore received a lot of attention. Given the potential fitness advantage provided by phenotypic noise in fluctuating environments, the possibility that it is directly subjected to evolutionary selection is being considered. For selection to act, phenotypic noise must differ between contemporary genotypes. Whether this is the case or not remains, however, unclear because phenotypic noise has very rarely been quantified in natural populations.

Results

Using automated image analysis, we describe here the phenotypic diversity of S. cerevisiae morphology at single-cell resolution. We profiled hundreds of quantitative traits in more than 1,000 cells of 37 natural strains, which represent various geographical and ecological origins of the species. We observed abundant trait variation between strains, with no correlation with their ecological origin or population history. Phenotypic noise strongly depended on the strain background. Noise variation was largely trait-specific (specific strains showing elevated noise for subset of traits) but also global (a few strains displaying elevated noise for many unrelated traits).

Conclusions

Our results demonstrate that phenotypic noise does differ quantitatively between natural populations. This supports the possibility that, if noise is adaptive, microevolution may tune it in the wild. This tuning may happen on specific traits or by varying the degree of global phenotypic buffering.

【 授权许可】

   
2013 Yvert et al.; licensee BioMed Central Ltd.

【 图 表 】

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