Transient filament occlusion of the middle cerebral artery in rats: does the reperfusion method matter 24 hours after perfusion?

BMC Neuroscience

Christoph Eschenfelder¹  Yi Zhao²  Johannes Meyne⁴  Thomas Herdegen²  Olav Jansen⁵  Günther Deuschl⁴  Xiao-Lei Shen³  Xiao-Yan Feng³  Fen Sun⁶  Jia-Jun Zhou⁶  Ulf R Jensen-Kondering⁵  Jian-Ren Liu⁶ 

[1] Boehringer Ingelheim Pharma GmbH & Co. KG, Ingelheim am Rhein, Germany;Institute of Pharmacology, University of Schleswig-Holstein, Campus Kiel, Christian-Albrechts-University Kiel, Kiel, Germany;Department of Neurology, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 639 Zhizaoju Road, Huangpu District, Shanghai, China;Department of Neurology, University of Schleswig-Holstein, Campus Kiel, Christian-Albrechts-University Kiel, Kiel, Germany;Institute of Neuroradiology, University of Schleswig-Holstein, Campus Kiel, Christian-Albrechts-University Kiel, Kiel, Germany;Department of Neurology, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China

关键词: Partial oxygen pressure;    Perfusion weighted imaging;    Diffusion weighted imaging;    Neurological deficits;    Unilateral Carotid reperfusion;    Bilateral Carotid reperfusion;    Reperfusion method;    Ischemia;    Middle cerebral artery occlusion;   

Others  :  1140629
DOI  :  10.1186/1471-2202-13-154

 received in 2012-09-20, accepted in 2012-12-19,  发布年份 2012

【 摘 要 】

Background

There are two widely used transient middle cerebral artery occlusion (MCAO) methods, which differ in the use of unilateral or bilateral carotid artery reperfusion (UNICAR and BICAR). Of the two methods, UNICAR is easier to perform. This study was designed to comprehensively compare the two reperfusion methods to determine if there are any differences in outcomes.

Results

The UNICAR and BICAR groups each included 9 rats. At baseline, the average pO₂ was 20.54 ± 9.35 and 26.43 ± 7.39, for the UNICAR and BICAR groups, respectively (P = 0.519). Changes in pO₂, as well as other physiological parameters measured within the ischemic lesion, were similar between the UNICAR and BICAR groups during 90 min of MCAO and the first 30 min of reperfusion (all P > 0.05). Furthermore, both the Bederson score and Garcia score, which are used for neurological assessment, were also similar (both P > 0.05). There were also no significant differences in T2WI lesion volume, DWI lesion volume, PWI lesion volume, or TTC staining infarct volume between the two groups (all P > 0.05).

Conclusion

UNICAR and BICAR have similar capability for inducing acute brain ischemic injury and can be considered interchangeable up to 24 hours after reperfusion.

【 授权许可】

   
2012 Liu et al.; licensee BioMed Central Ltd.

【 图 表 】

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