Virulence and genotypic characterization of Listeria monocytogenes isolated from vegetable and soil samples

BMC Microbiology

Suresh Kumar Dubey³  Durg Vijai Singh¹  Major Singh²  Dharmendra Kumar Soni³ 

[1] Infectious Disease Biology, Institute of Life Sciences, Bhubaneswar 751023, India;Indian Institute of Vegetable Research, Varanasi 221305, India;Department of Botany, Banaras Hindu University, Varanasi 221005, India

关键词: Multiplex PCR;    Virulence genes;    ERIC- and REP-PCR;    Serotype identification;   

Others  :  1170535
DOI  :  10.1186/s12866-014-0241-3

 received in 2014-03-13, accepted in 2014-09-02,  发布年份 2014

【 摘 要 】

Background

Listeria monocytogenes, a foodborne pathogen is ubiquitous to different environments including the agroecosystem. The organism poses serious public health problem. Therefore, an attempt has been made to gain further insight to their antibiotic susceptibility, serotypes and the virulence genes.

Results

Out of the 10 vegetables selected, 6 (brinjal, cauliflower, dolichos-bean, tomato, chappan-kaddu and chilli), 20 isolates (10%) tested positive for L. monocytogenes. The prevalence of the pathogen in the respective rhizosphere soil samples was 5%. Noticeably, L. monocytogenes was absent from only cabbage, broccoli, palak and cowpea, and also the respective rhizospheric soils. The 30 isolates?+?ve for pathogenicity, belonged to serogroup 4b, 4d or 4e, and all were positive for inlA, inlC, inlJ, plcA, prfA, actA, hlyA and iap gene except one (VC3) among the vegetable isolates that lacked the plcA gene. ERIC- and REP-PCR collectively revealed that isolates from vegetables and their respective rhizospheric soils had distinct PCR fingerprints.

Conclusions

The study demonstrates the prevalence of pathogenic L. monocytogenes in the selected agricultural farm samples. The increase in the number of strains resistant to ciprofloxacin and/or cefoxitin seems to pose serious public health consequences.

【 授权许可】

   
2014 Soni et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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