Increased IL-4 mRNA expression and poly-aromatic hydrocarbon concentrations from children with asthma

BMC Pediatrics

Majed S Alokail⁴  Mario S Clerici³  Abdul Khader Mohammed¹  Khalid Alkharfy⁴  Hossam Draz¹  Sherif Abd-Alrahman¹  Nasser M Al-Daghri² 

[1] Biomarkers Research Program, Biochemistry Department, College of Science, King Saud University, PO Box, 2455, 11451 Riyadh, Saudi Arabia;Prince Mutaib Chair for Biomarkers of Osteoporosis, Biochemistry Department, College of Science, King Saud University, 11451 Riyadh, Kingdom of Saudi Arabia;Chair of Immunology, Milano and Fondazione Don C. Gnocchi, IRCCS, University of Milano, Milano, Italy;Department of Clinical Pharmacy, College of Pharmacy, King Saud University, 1451 Riyadh, Saudi Arabia

关键词: Children;    Asthma;    Polycyclic aromatic hydrocarbons;    IL4;   

Others  :  1143938
DOI  :  10.1186/1471-2431-14-17

 received in 2013-06-09, accepted in 2014-01-14,  发布年份 2014

【 摘 要 】

Background

Asthma is the most common chronic childhood disease. Imbalance of cytokines released from T helper cells and environmental factors, such as exposure to poly-aromatic hydrocarbon (PAH), play pivotal roles in the pathogenesis of asthma. The aim of this study was to compare the mRNA expression patterns of Interleukin (IL)-4, interferon (IFN)-γ and Acyl Co A long chain 3 (ACSL3) in peripheral blood leukocytes of children with and without asthma. To correlate the obtained mRNA data with serum IL-4, IFN-γ and PAH levels. Further, to determine the effect of in vivo exposure to PAH on mRNA expression of IL-4, IFN-γ and ACSL3 genes in a rat model.

Methods

A total of 170 children below 16 years (85 pediatric asthma patients and 85 matched healthy controls) were randomly selected from the Riyadh Cohort, Saudi Arabia. Gene expression analysis was performed using qRTPCR. Serum IL-4, IFN-γ and PAH were measured using LINCOplex (human multiplex immunoassay kit) and HPLC respectively.

Results

IL-4 mRNA expression was significantly increased (P < 0.05) in children with asthma compared to healthy control group whereas no differences were observed for either IFN-γ or ACSL3 mRNA. Similarly, serum IL- 4 and PAHs concentration was significantly higher as well in children with asthma in whom IFN-γ was also significantly lower. Results obtained in rats showed that exposure to the benzopyrene prototype PAH resulted in a 2.6 fold (P < 0.001) increased IL-4 mRNA expression in blood.

Conclusion

Peripheral blood IL-4 mRNA levels, serum concentration of this cytokine are elevated in children with asthma. Also, elevated levels of PAH were observed in children with asthma. Additionally, PAH administration in rodents resulted in an increased IL-4 mRNA which is supposed to partly mediate the inflammatory response noted in asthma.

【 授权许可】

   
2014 Al-Daghri et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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