Real-time polymerase chain reaction analysis of MDM2 and CDK4 expression using total RNA from core-needle biopsies is useful for diagnosing adipocytic tumors

BMC Cancer

Taro Sasaki⁵  Akira Ogose⁵  Hiroyuki Kawashima⁵  Tetsuo Hotta⁵  Hiroshi Hatano⁴  Takashi Ariizumi⁴  Hajime Umezu¹  Riuko Ohashi¹  Tsuyoshi Tohyama²  Naohito Tanabe³  Naoto Endo⁵ 

[1] Division of Pathology, Niigata University Medical and Dental Hospital, Niigata, Japan

[2] Center of Molecular Biology and Cytogenetics, SRL, Inc, Tokyo, Japan

[3] Department of Health and Nutrition, Faculty of Human Life Studies, University of Niigata Prefecture, Niigata, Japan

[4] Departments of Orthopedic Surgery, Niigata Cancer Center Hospital, Niigata, Japan

[5] Division of Orthopedic Surgery, Niigata University Graduate School of Medical and Dental Sciences, 757-1, Asahimachi-dori, Niigata City, Niigata 951-8510, Japan

关键词: Real-time PCR;    CDK4;    MDM2;    Adipocytic tumors;    Atypical lipomatous tumor;    Liposarcoma;   

Others  :  855413
DOI  :  10.1186/1471-2407-14-468

 received in 2014-01-29, accepted in 2014-06-19,  发布年份 2014

【 摘 要 】

Background

Diagnosing adipocytic tumors can be challenging because it is often difficult to morphologically distinguish between benign, intermediate and malignant adipocytic tumors, and other sarcomas that are histologically similar. Recently, a number of tumor-specific chromosome translocations and associated fusion genes have been identified in adipocytic tumors and atypical lipomatous tumors/well-differentiated liposarcomas (ALT/WDL), which have a supernumerary ring and/or giant chromosome marker with amplified sequences of the MDM2 and CDK4 genes. The purpose of this study was to investigate whether quantitative real-time polymerase chain reaction (PCR) could be used to amplify MDM2 and CDK4 from total RNA samples obtained from core-needle biopsy sections for the diagnosis of ALT/WDL.

Methods

A series of lipoma (n = 124) and ALT/WDL (n = 44) cases were analyzed for cytogenetic analysis and lipoma fusion genes, as well as for MDM2 and CDK4 expression by real-time PCR. Moreover, the expression of MDM2 and CDK4 in whole tissue sections was compared with that in core-needle biopsy sections of the same tumor in order to determine whether real-time PCR could be used to distinguish ALT/WDL from lipoma at the preoperative stage.

Results

In whole tissue sections, the medians for MDM2 and CDK4 expression in ALT/WDL were higher than those in the lipomas (P < 0.05). Moreover, karyotype subdivisions with rings and/or giant chromosomes had higher MDM2 and CDK4 expression levels compared to karyotypes with 12q13-15 rearrangements, other abnormal karyotypes, and normal karyotypes (P < 0.05). On the other hand, MDM2 and CDK4 expression levels in core-needle biopsy sections were similar to those in whole-tissue sections (MDM2: P = 0.6, CDK4: P = 0.8, Wilcoxon signed-rank test).

Conclusion

Quantitative real-time PCR of total RNA can be used to evaluate the MDM2 and CDK4 expression levels in core-needle biopsies and may be useful for distinguishing ALT/WDL from adipocytic tumors. Thus, total RNA from core-needle biopsy sections may have potential as a routine diagnostic tool for other tumors where gene overexpression is a feature of the tumor.

【 授权许可】

   
2014 Sasaki et al.; licensee BioMed Central Ltd.

【 图 表 】

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