14-3-3β protein expression in eosinophilic meningitis caused by Angiostrongylus cantonensis infection

BMC Research Notes

Ming-Hong Tai¹  Susan Shin-Jung Lee²  Rachel Tsai¹  Chuan-Min Yen³  Yao-Shen Chen²  Yen-Lin Huang¹  Hung-Chin Tsai² 

[1] Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohisung, Taiwan, Republic of China;National Yang-Ming University, Taipei, Taiwan;Department of Parasitology and Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan

关键词: 14-3-3β protein;    Eosinophilic meningitis;    Angiostrongylus cantonensis;   

Others  :  1134467
DOI  :  10.1186/1756-0500-7-97

 received in 2013-03-22, accepted in 2014-02-11,  发布年份 2014

【 摘 要 】

Background

Angiostrongylus cantonensis is a parasite endemic in the Southeast Asian and Pacific regions. Humans are incidentally infected either by eating uncooked intermediate hosts or by consuming vegetables containing the living third-stage larvae. The 14-3-3β protein is a cerebrospinal fluid (CSF) marker of neuronal damage during the development of Creutzfeldt-Jakob disease. In addition, increased 14-3-3β protein is also found in CSF from patients with a variety of neurological disorders. The goal of this study is to determine the roles of serum/CSF14-3-3β protein in patients with eosinophilic meningitis.

Methods

In a cohort study among nine Thai laborers with eosinophilic meningitis due to eating raw snails (Pomacea canaliculata), we examined the CSF weekly while patients were still hospitalized and followed up the serum for 6 months. The levels of 14-3-3β protein in CSF were analyzed by western blot and an in-house 14-3-3β enzyme-linked immunosorbent assay (ELISA) measurement was established and tested in an animal model of eosinophilic meningitis.

Results

The elevated 14-3-3β level was detected in the CSF from eight out of nine (81%) patients After 2 weeks of treatment, all patients showed a declined level or cleared of 14-3-3β protein in the CSF. By developing an in-house ELISA for measurement of 14-3-3β protein, it was found that the serum 14-3-3β level was significantly increased in patients during initial visit. . This finding was consistent to the animal experiment result in which there was severe blood brain barrier damage three weeks after infection and increased 14-3-3β protein expression in the CSF and serum by western blot and in house ELISA. After treatment, the serum 14-3-3β level in meningitis patients was rapidly returned to normal threshold. There was a correlation between initial CSF 14-3-3β level with severity of headache (r = 0.692, p = 0.039), CSF pleocytosis (r = 0.807, p = 0.009) and eosinophilia (r = 0.798, p = 0.01) in the CSF of patients with eosinophilic meningitis (Spearman’s correlation test).

Conclusions

The serum 14-3-3β concentrations may constitute a useful marker for blood brain barrier damage severity and follow up in patients with eosinophilic meningitis caused by A. cantonensis.

【 授权许可】

   
2014 Tsai et al.; licensee BioMed Central Ltd.

【 图 表 】

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