Temperature-induced variation in gene expression burst size in metazoan cells

BMC Molecular Biology

Olivier Gandrillon¹  Guillaume Beslon³  Elodie Vallin¹  Sam Meyer⁴  Ophélie Arnaud² 

[1] Laboratoire de Biologie Moléculaire de la Cellule, Ecole Normale Supérieure de Lyon, CNRS, Université de Lyon, 46 Allée d’Italie, Lyon, 69007, France;Division of Genomic Technologies, RIKEN Center for Life Science Technologies, Yokohama, Japan;Inria Team Beagle, Inria Center Grenoble Rhône-Alpes, Montbonnot-Saint-Martin, France;INSA-Lyon, CNRS UMR5240 Microbiologie, Adaptation et Pathogénie, Université de Lyon, Lyon, 69622, France

关键词: Temperature;    Stochastic model;    Expression noise;   

Others  :  1234459
DOI  :  10.1186/s12867-015-0048-2

 received in 2015-03-09, accepted in 2015-11-10,  发布年份 2015

【 摘 要 】

Background

Gene expression is an inherently stochastic process, owing to its dynamic molecular nature. Protein amount distributions, which can be acquired by cytometry using a reporter gene, can inform about the mechanisms of the underlying microscopic molecular system.

Results

By using different clones of chicken erythroid progenitor cells harboring different integration sites of a CMV-driven mCherry protein, we investigated the dynamical behavior of such distributions. We show that, on short term, clone distributions can be quickly regenerated from small population samples with a high accuracy. On longer term, on the contrary, we show variations manifested by correlated fluctuation in the Mean Fluorescence Intensity. In search for a possible cause of this correlation, we demonstrate that in response to small temperature variations cells are able to adjust their gene expression rate: a modest (2 °C) increase in external temperature induces a significant down regulation of mean expression values, with a reverse effect observed when the temperature is decreased. Using a two-state model of gene expression we further demonstrate that temperature acts by modifying the size of transcription bursts, while the burst frequency of the investigated promoter is less systematically affected.

Conclusions

For the first time, we report that transcription burst size is a key parameter for gene expression that metazoan cells from homeotherm animals can modify in response to an external thermal stimulus.

【 授权许可】

   
2015 Arnaud et al.

【 图 表 】

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