Long term culture of MDCK strains alters chromosome content

BMC Research Notes

Doris Cassio¹ 

[1] Inserm UMR-S757, Orsay, 91405, France

关键词: Cell line drift;    Chromosome content;    Metaphase;    MDCK II;    MDCK I;    Madin-Darby Canine Kidney (MDCK) cells;   

Others  :  1142882
DOI  :  10.1186/1756-0500-6-162

 received in 2013-01-04, accepted in 2013-04-17,  发布年份 2013

【 摘 要 】

Background

The Madin-Darby Canine Kidney (MDCK) cell line and its different strains are widely used as models for studying epithelial simple polarity. Recently Dukes et al. [BMC Cell Biology 12:43, 2011] provided a useful guide to the different MDCK strains, with a directory of where to buy them. The present work focused on chromosome content of MDCK cells, a parameter often disregarded by researchers working with these cells.

Findings

Using a general and reliable method for obtaining high yield of metaphasic preparations, the chromosome content of MDCK, MDCK I, MDCK II obtained from reliable sources was determined after maintaining them in culture for various periods of time. Within two months significant changes were observed in the range and the mean number of chromosomes of MDCK I and MDCK II cells. MDCK II cells routinely cultured in six different laboratories were also examined. In some of these cultures the cells have considerably drifted as shown by a high scattering in their number of chromosomes.

Conclusion

These results entitle me to encourage researchers using MDCK cells obtained from reliable sources, to determine their chromosomal content upon receipt, to check this content after several passages, to use this feature to follow the possible drift of these cells, and finally to avoid working with cells maintained for more than two months in culture.

【 授权许可】

   
2013 Cassio; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

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