期刊论文详细信息
BMC Complementary and Alternative Medicine
Anti-hyperglycemic effects of three medicinal plants in diabetic pregnancy: modulation of T cell proliferation
Naim A Khan2  Kabirou Moutairou3  Mansourou Moudachirou4  Zouhair Tabka5  Anne-Marie Simonin2  Aziz Hichami1  Oussama Grissa5  Joachim Gbenou4  Akadiri Yessoufou3 
[1] INSERM U866, University of Bourgogne, 6 BD Gabriel, Dijon, 21000, France;University of Bourgogne, Physiology of Nutrition and Toxicology, INSERM UMR866, AgroSup/UB, Faculty of Life Sciences, 6 Boulevard Gabriel, Dijon, 21000, France;Laboratory of Cell Biology and Physiology, Department of Biochemistry and Cellular Biology, Faculty of Sciences and Techniques (FAST) and Institute of Applied Biomedical Sciences (ISBA), University of Abomey-Calavi, Cotonou, 01 BP 918, Benin;Laboratory of Pharmacognosy and Essential Oils (ISBA/FAST), University of Abomey-Calavi, Cotonou, 01 BP 918, Benin;Department of Physiology and Functional Exploration, University Hospital Farhat Hached, Sousse, 4000, Tunisia
关键词: Fatty acids;    T-cell proliferation;    Antioxidants;    Diabetic pregnancy;    Medicinal plants;   
Others  :  1230115
DOI  :  10.1186/1472-6882-13-77
 received in 2012-05-26, accepted in 2013-03-26,  发布年份 2013
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【 摘 要 】

Background

Populations in Africa mostly rely on herbal concoctions for their primarily health care, but so far scientific studies supporting the use of plants in traditional medicine remain poor. The present study was undertaken to evaluate the anti-hyperglycemic effects of Picralima nitida (seeds), Nauclea latifolia (root and stem) and Oxytenanthera abyssinica (leaves) commonly used, in diabetic pregnancy.

Methods

Pregnant wistar rats, rendered diabetic by multiple low injections of streptozotocin, were treated with selected plant extracts based on their antioxidant activities. Vitamin C concentrations, fatty acid compositions and phytochemical analysis of plants extracts were determined. Effect of selected plant extracts on human T cell proliferation was also analysed.

Results

All analysed plant extracts exhibited substantial antioxidant activities probably related to their content in polyphenols. Picralima nitida exhibited the highest antioxidant capacity. Ethanolic and butanolic extracts of Picralima nitida, butanolic extract of Nauclea latifolia and ethanolic extract of Oxytenanthera abyssinica significantly decreased hyperglycemia in the diabetic pregnant rats. Butanolic extract of Picralima, also appeared to be the most potent immunosuppressor although all of the analysed extracts exerted an immunosuppressive effect on T cell proliferation probably due to their linolenic acid (C18:3n-3) and/or alkaloids content. Nevertheless, all analysed plants seemed to be good source of saturated and monounsaturated fatty acids.

Conclusion

By having antioxidant, anti-hyperglycemic and immunosuppressive activities, these plants could be good candidates in the treatment of diabetes and diabetic pregnancy.

【 授权许可】

   
2013 Yessoufou et al.; licensee BioMed Central Ltd.

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【 参考文献 】
  • [1]World Health Organization: World health statistics. 2011, 170.
  • [2]Adzu B, Amos S, Amizan MB, Gamaniel K: Evaluation of the antidiarrheal effects of Zizyphus spina-christi stem bark in rats. Acta Trop 2003, 7:245-250.
  • [3]Bickii J, Tchouya GR, Tchouankeu JC, Tsamo E: Antimalarial activity in crude extracts of some Cameroonian medicinal plants. Afr J Tradit Complement Altern Med 2006, 4:107-111.
  • [4]Okokon JE, Antia BS, Igboasoiyi AC, Essien EE, Mbagwu HO: Evaluation of antiplasmodial activity of ethanolic seed extract of Picralima nitida. J Ethnopharmacol 2007, 111(Suppl 3):464-467.
  • [5]Taïwe GS, Bum EN, Talla E, Dimo T, Weiss N, Sidiki N, Dawe A, Moto FC, Dzeufiet PD, De Waard M: Antipyretic and antinociceptive effects of Nauclea latifolia root decoction and possible mechanisms of action. Pharm Biol 2011, 49(Suppl 1):15-25.
  • [6]Tchacondo T, Karou SD, Batawila K, Agban A, Ouro-Bang'na K, Anani KT, Gbeassor M, de Souza C: Herbal remedies and their adverse effects in tem tribe traditional medicine in Togo. Afr J Tradit Complement Altern Med 2011, 8(Suppl 1):45-60.
  • [7]Anand KK, Singh B, Chand D, Chandan BK, Gupta V: Effect of Zizyphus sativa leaves on blood glucose levels in normal and alloxan-diabetic rats. J Ethnopharmacol 1989, 27:121-125.
  • [8]Tschesche R, Kaubmann EU: The Alkaloids. Edited by Manske R. New York: HF AdemicPress; 1975:165-205.
  • [9]Benammar C, Hichami A, Yessoufou A, Simonin AM, Belarbi M, Allali H, Khan NA: Zizyphus lotus L. (Desf.) modulates antioxidant activity and human T-cell proliferation. BMC Complement Altern Med 2010, 10(54):1-9.
  • [10]Akoègninou A, van der Burg WJ, van der Maesen LJG, Adjakidjè V, Essou JP, Sinsin B, Yédomonhan H: Flore Analytique du Bénin. Wagenigen: Backhuys Publisher; 2006:1034.
  • [11]Duwiejua M, Woode E, Obiri DD: Pseudo-akuammigine, an alkaloid from Picralima nitida seeds, has anti-inflammatory and analgesic actions in rats. J Ethnopharmacol 2002, 81(suppl 1):73-79.
  • [12]Fakeye TO, Itiola OA, Odelola HA: Evaluation of the antimicrobial property of the stem bark of Picralima nitida (Apocynaceae). Phytother Res 2000, 14(suppl 5):368-370.
  • [13]Mathew OJ, Ogochukwu AM, Michael UC: Antiulcer activity of methanolic extract and fractions of Picralima nitida seeds (Apocynacaea) in rats. Asian Pac J Trop Med 2011, 4:13-15.
  • [14]Igboasoiyi AC, Essien EE, Eseyin OA, Ubam G: Screening of the seed of Picralima nitida for hypoglycaemic activity. Pak J Biol Sci 2007, 10(suppl 5):828-830.
  • [15]Aguwa CN, Ukwe CV, Inya-Agha SI, Okonta JM: Antidiabetic effect of Picralima nitida aqueous seed extract in experimental rabbit model. J Nat Remedies 2001, 1(Suppl 2):135-139.
  • [16]Inya-Agha SI, Ezea SC, Odukoya OA: Evaluation of Picralima nitida hypoglycaemic activity, toxicity and analytical standards. Int J Pharmacol 2006, 2(Suppl 5):576-580.
  • [17]Atègbo JM, Grissa O, Yessoufou A, Hichami A, Dramane KL, Moutairou K, Miled A, Grissa A, Jerbi M, Tabka Z, Khan NA: Modulation of Adipokines and Cytokines in Gestational Diabetes and Macrosomia. J Clin Endocrinol Metab 2006, 91(suppl 10):4137-4143.
  • [18]Grissa O, Atègbo JM, Yessoufou A, Tabka Z, Miled A, Moutairou K, Jerbi M, Dramane KL, Prost J, Hichami A, Khan NA: Antioxidant status and circulating lipids are altered in human gestational diabetes and macrosomia. Transl Res 2007, 150(suppl 3):164-171.
  • [19]Guermouche B, Yessoufou A, Soulaimann N, Merzouk H, Moutairou K, Hichami A, Khan NA: (N-3) Fatty Acids Modulate T-Cell Calcium Signalling in Obese Macrosomic Rats. Obes Res 2004, 12:1744-1753.
  • [20]Khan NA, Yessoufou A, Kim M, Hichami A: N-3 fatty acids modulate TH1 and TH2 dichotomy in diabetic pregnancy and macrosomia. J Autoim 2006, 26:268-277.
  • [21]Yessoufou A, Moutairou K: Maternal diabetes in pregnancy: early and long-term outcomes on the offspring and the concept of "metabolic memory". Exp Diabetes Res 2011, 2011:1-12.
  • [22]Yessoufou A, Soulaimann N, Merzouk S, Moutairou K, Ahissou H, Prost J, Simonin AM, Hichami A, Khan NA: N-3 Fatty Acids Modulate Antioxidant Status in Diabetic Rats and their Macrosomic Offspring. Int J Obes 2006, 30:739-750.
  • [23]Van Assche FA, Holemans K, Aerts L: Long-term consequences for offspring of diabetes during pregnancy. Brit Med Bull 2001, 60:173-182.
  • [24]Herold KC, Vezys V, Sun Q, Viktora D, Seung E, Reiner S, Brown DR: Regulation of cytokine production during development of autoimmune diabetes induced with multiple low doses of streptozotocin. J Immunol 1996, 156:3521-3527.
  • [25]Yessoufou A, Hichami A, Besnard P, Moutairou K, Khan NA: PPARα deficiency increases the risk of maternal abortion and neonatal mortality in murine pregnancy with or without diabetes mellitus: modulation of T cell differentiation. Endocrinology 2006, 147:4410-4418.
  • [26]Sathyapalan T, Atkin SL: Is there a role for immune and anti-inflammatory therapy in type 2 diabetes? Minerva Endocrinol 2011, 36(suppl 2):147-156.
  • [27]Bendich A: Physiological role of antioxidants in the immune system. J Dairy Sci 1993, 76:2789-2794.
  • [28]Keita A, Mariko E, Haidara TK: Etude de l’activité hypoglycémiante des feuilles de Sclerocarya birrea (A. Rich) Hochst. (Anacardiaceae): action de la fraction butanolique de l’extrait aqueux. Pharm Med Trad Afr 1998, 10:16-25.
  • [29]Roe JH, Kuether CA: The determination of ascorbic acid in whole blood and urine through the 2,4-dinitrophenylhydrazine derivatives of dehydroascorbic acid. J Biol Chem 1943, 147:399-407.
  • [30]Blache D, Prost M: Free radical attack: biological test for human resistance capability. In A lunar-based chemical analysis laboratory”. Edited by Ponnamperurna C, Gehrke CW. Hampton, VA: Deepak A; 1992:82-98.
  • [31]Houghton PJ, Amala R: Laboratory Handbook for the Fractionation of Natural Extracts. 1st edition. New York: Chapman and Hall; 1998:244. 199p
  • [32]Bligh EG, Dyer WJ: A rapid method of total lipid extraction and purification. Can J Biochem Physiol 1995, 37:911-917.
  • [33]Solver HT, Lanza E: Quantitative analysis of food fatty acids by capillary gas chromatography. J Am Oil Chem Soc 1979, 56:933-943.
  • [34]Rabinovitch A: Immunoregulatory and cytokine imbalances in the pathogenesis of IDDM: therapeutic intervention by immunostimulation? Diabetes 1994, 43(suppl 5):613-621.
  • [35]Muller A, Schott-Ohly P, Dohle C, Gleichmann H: Differential regulation of Th1-type and Th2-type cytokine profiles in pancreatic islets of C57BL/6 and BALB/c mice by multiple low doses of streptozotocin. Immunobiology 2002, 205:35-50.
  • [36]Rossini AA, Williams RM, Appel MC, Like AA: Complete protection from low-dose streptozotocin-induced diabetes in mice. Nature 1978, 276:182-184.
  • [37]Vercheval M, De Hertogh R, Pampfer S, Vanderheyden I, Michiels B, De Bernardi P, De Meyer R: Experimental diabetes impairs rat development during the preimplantation period. Diabetologia 1990, 33:187-191.
  • [38]Lopez-Soldado I, Herrera E: Different diabetogenic response to moderate doses of streptozotocin in pregnant rats, and its long-term consequences in the offspring. Exp Diabesity Res 2003, 4:107-118.
  • [39]Aderibigbe AO, Emudianughe TS, Lawal BA: Antihyperglycaemic effect of Mangifera indica in rat. Phytother Res 1999, 13(Suppl 6):504-507.
  • [40]Smirnoff N: L-ascorbic acid biosynthesis. Vitam. Horm. A Review 2001, 61:241-266.
  • [41]Borgi W, Recio MC, Ríos JL, Chouchane N: Anti-inflammatory and analgesic activities of flavonoid and saponin fractions from Zizyphus lotus (L) Lam. South Afr J Bot 2008, 74:320-324.
  • [42]Lenucci MS, Cadinu D, Taurino M, Piro G, Dalessandro G: Antioxidant composition in cherry and high-pigment tomato cultivars. J Agric Food Chem 2006, 54:2606-2613.
  • [43]Lamien-Meda A, Lamien CE, Compaoré MM, Meda RN, Kiendrebeogo M, Zeba B, Millogo JF, Nacoulma OG: Polyphenol content and antioxidant activity of fourteen wild edible fruits from Burkina Faso. Molecules 2008, 13:581-594.
  • [44]Domingues A, Sartori A, Valente LM, Golim MA, Siani AC, Viero RM: Uncaria tomentosa aqueous-ethanol extract triggers an immunomodulation toward a Th2 cytokine profile. Phytother Res 2011, 25(Suppl 8):1229-1235.
  • [45]Calder PC, Krauss-Etschmann S, de Jong EC, Dupont C, Frick JS, Frokiaer H, Heinrich J, Garn H, Koletzko S, Lack G, Mattelio G, Renz H, Sangild PT, Schrezenmeir J, Stulnig TM, Thymann T, Wold AE, Koletzko B: Early nutrition and immunity - progress and perspectives. Br J Nutr 2006, 96:774-790.
  • [46]Khan NA: Polyunsaturated fatty acids in the modulation of T-cell signalling. Prostaglandins Leukot Essent Fatty Acids 2010, 82:179-187.
  • [47]da Conceição AO, Rossi MH, de Oliveira FF, Takser L, Lafond J: Genipa americana (Rubiaceae) fruit extract affects mitogen-activated protein kinase cell pathways in human trophoblast-derived BeWo cells: implications for placental development. J Med Food 2011, 14(5):483-494.
  • [48]Bacher N, Tiefenthaler M, Sturm S, Stuppner H, Ausserlechner MJ, Kofler R, Konwalinka G: Oxindole alkaloids from Uncaria tomentosa induce apoptosis in proliferating, G0/G1-arrested and bcl-2-expressing acute lymphoblastic leukaemia cells. Br J Haematol 2006, 132(Suppl 5):615-622.
  • [49]Traore F, Gasquet M, Laget M, Guiraud H, Di Giorgio C, Azas N, Doumbo O, Timon-David P: Toxicity and genotoxicity of antimalarial alkaloid rich extracts derived from Mitragyna inermis O. Kuntze and Nauclea latifolia. Phytother Res 2000, 14(8):608-611.
  • [50]Mesaik MA, Halim SA, Ul-Haq Z, Choudhary MI, Shahnaz S, Ayatollahi SA, Murad S, Ahmad A: Immunosuppressive activity of buxidin and E-buxenone from Buxus hyrcana. Chem Biol Drug Des 2010, 75(3):310-317.
  • [51]Yuan D, Ma B, Yang JY, Xie YY, Wang L, Zhang LJ, Kano Y, Wu CF: Anti-inflammatory effects of rhynchophylline and isorhynchophylline in mouse N9 microglial cells and the molecular mechanism. Int Immunopharmacol 2009, 9(Suppl 13–14):1549-1554.
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