Ophthalmic infections in children presenting to Angkor Hospital for Children, Siem Reap, Cambodia

BMC Research Notes

Christopher M Parry¹  Nicholas PJ Day¹  H Rogier van Doorn³  Vu thi Ty Hang³  Stephen Baker²  Duy Pham Thanh³  Kate Emary¹  Nicole Stoesser¹  Rachel Bousfield¹  Catrin E Moore¹  Sona Soeng⁴  Channy Soeng⁴  Paul Turner¹  Phara Khauv⁴ 

[1] Centre for Tropical Medicine, Nuffield Department of Medicine, University of Oxford, Oxford, UK;The London School of Hygiene and Tropical Medicine, London, United Kingdom;The Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme, Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam;Angkor Hospital for Children, Siem Reap, Cambodia

关键词: Chlamydia;    Infection;    Ophthalmic;    Paediatric;   

Others  :  1125704
DOI  :  10.1186/1756-0500-7-784

 received in 2013-12-20, accepted in 2014-10-21,  发布年份 2014

【 摘 要 】

Background

Ophthalmic infections cause significant morbidity in Cambodian children but aetiologic data are scarce. We investigated the causes of acute eye infections in 54 children presenting to the ophthalmology clinic at Angkor Hospital for Children, Siem Reap between March and October 2012.

Findings

The median age at presentation was 3.6 years (range 6 days – 16.0 years). Forty two patients (77.8%) were classified as having an external eye infection, ten (18.5%) as ophthalmia neonatorum, and two (3.7%) as intra-ocular infection. Organisms were identified in all ophthalmia neonatorum patients and 85.7% of patients with an external eye infection. Pathogens were not detected in either of the intra-ocular infection patients. Most commonly isolated bacteria were Staphylococcus aureus (23 isolates), coagulase-negative staphylococci (13), coliforms (7), Haemophilus influenzae/parainfluenzae (6), Streptococcus pneumoniae (4), and Neisseria gonorrhoeae (2). Chlamydia trachomatis DNA was detected in 60% of swabs taken from ophthalmia neonatorum cases.

Conclusions

This small study demonstrates the wide range of pathogens associated with common eye infections in Cambodian children. The inclusion of molecular assays improved the spectrum of detectable pathogens, most notably in neonates.

【 授权许可】

   
2014 Khauv et al.; licensee BioMed Central Ltd.

【 参考文献 】

• [1]Thomson I: A clinic based survey of blindness and eye disease in Cambodia. Br J Ophthalmol 1997, 81(7):578-580.
• [2]Jackson H, Foster A: Causes of blindness in northwest Cambodia. Ophthalmic Epidemiol 1997, 4(1):27-32.
• [3]Rutzen AR, Ellish NJ, Schwab L, Graham PJ, Pizzarello LD, Hemady RK, Maldonado MJ: Blindness and eye disease in Cambodia. Ophthalmic Epidemiol 2007, 14(6):360-366.
• [4]Dumler JS, Funke G, Janda JM, Nachamkin I, Versalovic J: Bacteriology. In Manual of Clinical Microbiology. Volume 1. 9th edition. Edited by Murray PR, Baron EJ, Jorgensen JH, Landry ML, Pfaller MA. Washington DC: ASM Press; 2007:272-1074.
• [5]CLSI: Performance Standards for Antimicrobial Susceptibility Testing; Seventeenth Informational Supplement. CLSI document M100-S23. Clinical and Laboratory Standards Institute: Wayne, PA; 2013.
• [6]Jalal H, Stephen H, Curran MD, Burton J, Bradley M, Carne C: Development and validation of a rotor-gene real-time PCR assay for detection, identification, and quantification of Chlamydia trachomatis in a single reaction. J Clin Microbiol 2006, 44(1):206-213.
• [7]Jansen RR, Schinkel J, Koekkoek S, Pajkrt D, Beld M, De Jong MD, Molenkamp R: Development and evaluation of a four-tube real time multiplex PCR assay covering fourteen respiratory viruses, and comparison to its corresponding single target counterparts. J Clin Virol 2011, 51(3):179-185.
• [8]Van Doornum GJ, Guldemeester J, Osterhaus AD, Niesters HG: Diagnosing herpesvirus infections by real-time amplification and rapid culture. J Clin Microbiol 2003, 41(2):576-580.
• [9]Sergiwa A, Pratt BC, Eren E, Sunona TC, Hart CA: Ophthalmia neonatorum in Bangkok: the significance of Chlamydia trachomatis. Ann Trop Paediatr 1993, 13(3):233-236.
• [10]Shah A, Sachdev A, Coggon D, Hossain P: Geographic variations in microbial keratitis: an analysis of the peer-reviewed literature. Br J Ophthalmol 2011, 95(6):762-767.
• [11]Young AL, Leung KS, Tsim N, Hui M, Jhanji V: Risk factors, microbiological profile, and treatment outcomes of pediatric microbial keratitis in a tertiary care hospital in Hong Kong. Am J Ophthalmol 2013, 156(5):1040-1044. e1042
• [12]Lee YS, Tan HY, Yeh LK, Lin HC, Ma DH, Chen HC, Chen SY, Chen PY, Hsiao CH: Pediatric microbial keratitis in Taiwan: clinical and microbiological profiles, 1998-2002 versus 2008-2012. Am J Ophthalmol 2014, 157(5):1090-1096.
• [13]Jhanji V, Naithani P, Lamoureux E, Agarwal T, Sharma N, Vajpayee RB: Immunization and nutritional profile of cases with atraumatic microbial keratitis in preschool age group. Am J Ophthalmol 2011, 151(6):1035-1040. e1032
• [14]Pagnarith Y, Kumar V, Thaipadungpanit J, Wuthiekanun V, Amornchai P, Sin L, Day NP, Peacock SJ: Emergence of pediatric melioidosis in Siem Reap, Cambodia. Am J Trop Med Hyg 2010, 82(6):1106-1112.
• [15]Chheng K, Tarquinio S, Wuthiekanun V, Sin L, Thaipadungpanit J, Amornchai P, Chanpheaktra N, Tumapa S, Putchhat H, Day NP, Peacock SJ: Emergence of community-associated methicillin-resistant Staphylococcus aureus associated with pediatric infection in Cambodia. PLoS One 2009, 4(8):e6630.
• [16]Vernel-Pauillac F, Ratsima EH, Guillard B, Goursaud R, Lethezer C, Hem S, Merien F, Goarant C: Correlation between antibiotic susceptibilities and genotypes in Neisseria gonorrhoeae from different geographical origins: determinants monitoring by real-time PCR as a complementary tool for surveillance. Sex Transm Infect 2010, 86(2):106-111.
• [17]Olsen B, Pham TL, Golparian D, Johansson E, Tran HK, Unemo M: Antimicrobial susceptibility and genetic characteristics of Neisseria gonorrhoeae isolates from Vietnam, 2011. BMC Infect Dis 2013, 13:40. BioMed Central Full Text