Zinc treatment ameliorates diarrhea and intestinal inflammation in undernourished rats

BMC Gastroenterology

Reinaldo B Oriá¹  Sean R Moore⁶  Richard L Guerrant³  Ronaldo A Ribeiro¹  Aldo Ângelo M Lima¹  Cibele BM de Carvalho⁵  Carlos Emanuel C Magalhães⁴  Karoline S Aragão¹  Ítalo L Figueiredo⁴  Priscila B Frota⁴  Said Gonçalves C Fonseca²  Camila AA de Queiroz⁴ 

[1] Department of Physiology and Pharmacology and Institute of Biomedicine, School of Medicine, Federal University of Ceara, Rua Cel. Nunes de Melo, 1315, Fortaleza, CE, Brazil;Department of Pharmacy, Faculty of Pharmacy, Odontology, and Nursing, Federal University of Ceara, Rua Capitão Francisco Pedro, 1210, Fortaleza, CE, Brazil;Center for Global Health, Division of Infectious Diseases and International Health, University of Virginia, Charlottesville, VA, USA;Laboratory of the Biology of Tissue Healing, Ontogeny and Nutrition, Department of Morphology and Institute of Biomedicine, School of Medicine, Federal University of Ceara, Rua Cel. Nunes de Melo, 1315, Fortaleza, CE, Brazil;Department of Microbiology and Institute of Biomedicine, School of Medicine, Federal University of Ceara, Rua Cel. Nunes de Melo, 1315, Fortaleza, CE, Brazil;Division of Gastroenterology, Hepatology, & Nutrition, Cincinnati Children’s Hospital Medical Center, Cincinnati, OH, USA

关键词: Bacterial translocation;    Inflammation;    Diarrhea;    Malnutrition;    Zinc;   

Others  :  1121862
DOI  :  10.1186/1471-230X-14-136

 received in 2014-03-13, accepted in 2014-07-28,  发布年份 2014

【 摘 要 】

Background

WHO guidelines recommend zinc supplementation as a key adjunct therapy for childhood diarrhea in developing countries, however zinc’s anti-diarrheal effects remain only partially understood. Recently, it has been recognized that low-grade inflammation may influence stunting. In this study, we examined whether oral zinc supplementation could improve weight, intestinal inflammation, and diarrhea in undernourished weanling rats.

Methods

Rats were undernourished using a northeastern Brazil regional diet (RBD) for two weeks, followed by oral gavage with a saturated lactose solution (30 g/kg) in the last 7 days to induce osmotic diarrhea. Animals were checked for diarrhea daily after lactose intake. Blood was drawn in order to measure serum zinc levels by atomic absorption spectroscopy. Rats were euthanized to harvest jejunal tissue for histology and cytokine profiles by ELISA. In a subset of animals, spleen samples were harvested under aseptic conditions to quantify bacterial translocation.

Results

Oral zinc supplementation increased serum zinc levels following lactose-induced osmotic diarrhea. In undernourished rats, zinc improved weight gain following osmotic diarrhea and significantly reduced diarrheal scores by the third day of lactose intake (p < 0.05), with improved jejunum histology (p < 0.0001). Zinc supplementation diminished bacterial translocation only in lactose-challenged undernourished rats (p = 0.03) compared with the untreated challenged controls and reduced intestinal IL-1β and TNF-α cytokines to control levels.

Conclusion

Altogether our findings provide novel mechanisms of zinc action in the setting of diarrhea and undernutrition and support the use of zinc to prevent the vicious cycle of malnutrition and diarrhea.

【 授权许可】

   
2014 de Queiroz et al.; licensee BioMed Central Ltd.

【 图 表 】

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