Gateway-compatible tissue-specific vectors for plant transformation

BMC Research Notes

Lucia C Strader¹  Elizabeth M Frick¹  Marta Michniewicz¹ 

[1] Department of Biology, Washington University, St. Louis 63130, MO, USA

关键词: Tissue-specific;    Gateway technology;    Cloning vectors;   

Others  :  1135506
DOI  :  10.1186/s13104-015-1010-6

 received in 2014-07-25, accepted in 2015-02-11,  发布年份 2015

【 摘 要 】

Background

Understanding regulation of developmental events has increasingly required the use of tissue-specific expression of diverse genes affecting plant growth and environmental responses.

Findings

To allow for cloning of presumptive promoters with tissue-specific activities, we created two plant expression vectors with multiple cloning sites upstream of a Gateway cassette for expression of either untagged or YFP-tagged genes of interest. For fast and easy tissue-specific expression of desired genes, we further developed an initial set of Gateway-compatible tissue-specific gene expression vectors that allow for the expression of YFP-tagged or untagged proteins driven by the ALCOHOL DEHYDROGENASE1, CHLOROPHYLL A/B BINDING PROTEIN 1, COBRA LIKE1, EXPANSIN7, LATERAL ORGAN BOUNDARIES-DOMAIN 16, SCARECROW, UBIQUITIN10, and WOODEN LEG upstream regulatory regions.

Conclusions

These vectors provide an invaluable resource to the plant community, allowing for rapid generation of a variety of tissue-specific expression constructs.

【 授权许可】

   
2015 Michniewicz et al.; licensee BioMed Central.

【 图 表 】

Figure 1.

Figure 2.

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