Antifungal and antioxidant activity of Crassocephalum bauchiense (Hutch.) Milne-Redh ethyl acetate extract and fractions (Asteraceae)

BMC Research Notes

Jules-Roger Kuiate¹  Monique O Kamtchueng¹  Guy SS Njateng¹  Rosalie A Ngono Ngane²  Raymond Simplice Mouokeu³ 

[1] Laboratory of Microbiology and Antimicrobial Substances, Faculty of Sciences, P.O. Box 67, Dschang, Cameroon;Laboratory of Biochemistry, Faculty of Sciences, P.O. Box 24157, Douala, Cameroon;Laboratory of Microbiology and Food Quality Control, Institute of Fisheries and Aquatic Sciences, University of Douala, P.O. Box 7236, Douala, Cameroon

关键词: Asteraceae;    Crassocephalum bauchiense;    Antioxidant activity;    Antifungal activity;   

Others  :  1133433
DOI  :  10.1186/1756-0500-7-244

 received in 2013-03-07, accepted in 2014-04-09,  发布年份 2014

【 摘 要 】

Background

Crassocephalum bauchiense is a flowering plant, found in the West Region of Cameroon. Previous studied has highlighted the antibacterial and the dermal toxicological safety as well as the immunomodulatory activities of the ethyl acetate extract of its dry leaves. As an extension of the previous researches, the current work has been undertaken to evaluate the in vitro antifungal and antioxidant activities of C. bauchiense dried leaves ethyl acetate extract and fractions.

Methods

The extract was obtained by maceration in ethyl acetate and further fractionated into six fractions labeled F₁ to F₆ by flash chromatography. The antifungal activity of the extract and fractions against yeasts and dermatophytes was evaluated using broth microdilution method. Antioxidant activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide (NO) and β-carotene - linoleic acid assays.

Results

The extract (MIC = 0.125 - 4 mg/ml) was found to be more active on dermatophytes and yeasts compared to the fractions. The ethyl acetate extract and fractions exhibited strong scavenging activity on DPPH (CI₅₀ = 28.57 - 389.38 μg/ml). The fractions F₃ and F₆ expressed best antioxidant activity on DPPH radicals compared to the crude extract.

Conclusion

The results of these findings clearly showed that C. bauchiense ethyl acetate extract has a significant antifungal and antioxidant activity. It is therefore a source of active compounds that might be used as antifungal and antioxidant agents.

【 授权许可】

   
2014 Mouokeu et al.; licensee BioMed Central Ltd.

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