Mitogen-activated protein kinase Hog1 is activated in response to curcumin exposure in the budding yeast Saccharomyces cerevisiae

BMC Microbiology

Raghuvir Singh Tomar¹  Shivani Baranwal¹  Mayur Jankiram Thakare¹  Vikash Singh¹  Gajendra Kumar Azad² 

[1] Laboratory of Chromatin Biology, Department of Biological Sciences, Indian Institute of Science Education and Research, Bhopal 462023, India;Current address: Department of Genetics, Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel

关键词: Mitogen-activated protein kinases;    Glycerol-3-phosphate dehydrogenase 1 (GPD1);    Hog1 phosphorylation;    HOG pathway;    Curcumin;    Yeast drug response;   

Others  :  1090573
DOI  :  10.1186/s12866-014-0317-0

 received in 2014-07-29, accepted in 2014-12-05,  发布年份 2014

【 摘 要 】

Background

Curcumin (CUR), an active polyphenol derived from the spice turmeric, has been traditionally used for centuries in ancient Indian medicine to treat a number of diseases. The physiological effects of CUR have been shown to be diverse; however, the target molecules and pathways that CUR affects have yet to be fully described.

Results

Here, we demonstrate for the first time that the budding yeast mitogen-activated protein kinase (MAPK) Hog1 is essential for the response to CUR. Moreover, CUR-induced Hog1 phosphorylation was rescued by supplementation of iron to the growth medium. Hog1 was rapidly phosphorylated upon CUR treatment, but unlike the response to hyperosmotic shock (0.8 M NaCl), it remains activated for an extended period of time. A detailed analysis of HOG pathway mutants revealed that Pbs2p, Ptc2p, and Ssk2p are required for optimal CUR-induced Hog1 phosphorylation. We also observed a Hog1 dependent transcriptional response to CUR treatment that involved the up-regulation of glycerol-3-phosphate dehydrogenase 1 (GPD1), a factor that is essential for the hyperosmotic stress response.

Conclusions

Our present finding revealed the role of Hog1 MAPK in regulation of CUR-induced transcriptional response. We anticipate that our finding will enhance the understanding on the molecular mode of action of CUR on S. cerevisiae.

【 授权许可】

   
2014 Azad et al.; licensee BioMed Central.

【 图 表 】

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