Antioxidant and phytochemical analysis of Ranunculus arvensis L. extracts

BMC Research Notes

Salman Akbar Malik⁴  Asma Saeed¹  Ayaz Ahmad²  Amjad Ali³  Muhammad Zeeshan Bhatti⁴ 

[1] Department of Biological Sciences, Gomal University, Dera Ismail Khan 29050, Pakistan;Department of Biotechnology, Abdul Wali Khan University, Mardan 23200, Pakistan;Institute of Biomedical Sciences, School of Life Science, East China Normal University, 500 Dongchuan Road, Shanghai 200241, People’s Republic of China;Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan

关键词: HPLC;    Flavonoid content;    Phenolic content;    Ranunculus arvensis;    Antioxidant;   

Others  :  1231635
DOI  :  10.1186/s13104-015-1228-3

 received in 2014-09-09, accepted in 2015-06-14,  发布年份 2015

【 摘 要 】

Background

Ranunculus arvensis L. (R. arvensis) has long been used to treat a variety of medical conditions such as arthritis, asthma, hay fever, rheumatism, psoriasis, gut diseases and rheumatic pain. Here, we screened R. arvensis for antioxidant activity, phytochemical and high performance liquid chromatography (HPLC) analyses.

Methods

The chloroform, chloroform:methanol, methanol, methanol:acetone, acetone, methanol:water and water extracts of R. arvensis were examined for DPPH (1, 1-diphenyl-2-picrylhydrazyl) free radical scavenging assay, hydrogen peroxide scavenging assay, phosphomolybdenum assay, reducing power assay, flavonoid content, phenolic content and high performance liquid chromatography analysis.

Results

Significant antioxidant activity was displayed by methanol extract (IC₅₀34.71 ± 0.02) in DPPH free radical scavenging assay. Total flavonoids and phenolics ranged 0.96–6.0 mg/g of extract calculated as rutin equivalent and 0.48–1.43 mg/g of extract calculated as gallic acid equivalent respectively. Significant value of rutin and caffeic acid was observed via high performance liquid chromatography.

Conclusions

These results showed that extracts of R. arvensis exhibited significant antioxidant activities. Moreover, R. arvensis is a rich source of rutin, flavonoids and phenolics.

【 授权许可】

   
2015 Bhatti et al.

【 图 表 】

Figure1.

Figure2.

【 参考文献 】

• [1]Orak M, Ustundag M, Guloglu C, Tas M, Baylan B: A skin burn associated with Ranunculus arvensis. Ind J Dermatol. 2009, 54:19-20.
• [2]Akbulut S, Semur H, Kose O, Ozhasenekler A, Celiktas M, Basbug M, et al.: Phytocontact dermatitis due to Ranunculus arvensis mimicking burn injury: report of three casas and literature review. Intern J Emerg Med. 2011, 4:1-5. BioMed Central Full Text
• [3]Fabri RL, Nogueira MS, Braga FG, Coimbra ES, Scio E: Mitracarpus frigidus aerial parts exhibited potent antimicrobial, antileishmanial, and antioxidant effects. Bioresour Technol 2009, 100:428-433.
• [4]Kanwal S, Ullah N, Haq IU, Afzal I, Mirza B: Antioxidant, antitumor activities and phytochemical investigation of Hedra neplalensis K. Koch an important medicinal plant from Pakistan. Pak J Bot 2011, 43:85-89.
• [5]Boligon AA, Pereira RP, Feltrin AC, Machado MM, Janovik V, Rocha JBT, et al.: Antioxidant activities of flavonol derivatives from the leaves and stem bark of Scutia buxifolia Reiss. Bioresour Technol 2009, 100:6592-6598.
• [6]Sikder MAA, Rahman MA, Islam MR, Abul KM, Kiasar MA, Rahman MS, et al.: In-vitro antioxidant, reducing power, free radical scavenging and membrane stabilizing activities of Spilanthes calva. Bangladesh Pharmaceut J. 2010, 13:63-67.
• [7]Blazquez S, Olmos E, Hernandez JA, Fernandez-Garcıa N, Fernandez JA, Piqueras A: Somatic embryogenesis in saffron (Crocus sativus L.) Histological differentiation and implication of some components of the antioxidant enzymatic system. Plant Cell Tiss Organ Cult 2009, 97:49-57.
• [8]Zur I, Dubas E, Krzewska M, Janowiak F, Hura K, Pociecha E et al (2014) Antioxidant activity and ROS tolerance in triticale (xTriticosecale Wittm.) anthers affect the efficiency of microspore embryogenesis. Plant Cell Tiss Organ Cult. doi:10.1007/s11240-014-0515-3
• [9]Li L, Yi H: Effect of sulfur dioxide on ROS production, gene expression and antioxidant enzyme activity in Arabidopsis plants. Plant Physiol Biochem 2012, 58:46-53.
• [10]Hajaji HE, Lachkar N, Alaoui K, Cherrah Y, Farah A, Ennabili A, et al.: Antioxidant activity, phytochemical screening, and total phenolic content of extracts from three genders of carob tree barks growing in Morocco. Arabian J Chem 2011, 4:321-324.
• [11]Voravuthikunchaia SP, Kanchanapoomb T, Sawangjaroena N, Towatana NH: Antioxidant, antibacterial and antigiardial activities of Walsura robusta Roxb. Nat Prod Res 2010, 24:813-824.
• [12]Gill SS, Tuteja N: Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 2010, 48:909-930.
• [13]Miladi S, Damak M: In-vitro antioxidant activities of Aloe vera leaf skin extracts. J Soc Chim Tunisie 2008, 10:101-109.
• [14]Velasco P, Francisco M, Moreno DA, Ferreres F, García-Viguera C, Cartea ME: Phytochemical fingerprinting of vegetable Brassica oleracea and Brassica napus by simultaneous identification of glucosinolates and phenolics. Phytochem Anal 2011, 22:144-152.
• [15]Shah SMM, Sadiq A, Shah SMH, Ullah F: Antioxidant, total phenolic contents and antinociceptive potential of Teucrium stocksianum methanolic extract in different animal models. BMC Complement Altern Med. 2014, 14:181. BioMed Central Full Text
• [16]Kulisic T, Radonic A, Katalinic V, Milos M: Use of different methods for testing antioxidative activity of oregano essential oil. Food Chem 2004, 85:633-640.
• [17]Ruch RJ, Cheng SJ, Klaunig JE: Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis 1989, 10:1003-1008.
• [18]Prieto P, Pineda M, Aguliar M: Spectrophotometric quantitation of antioxidant capacity through the formation of phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem 1999, 269:337-341.
• [19]Oyaizu M: Studies on product of browning reaction prepared from glucose amine. Japanese J Nutr 1986, 44:307-315.
• [20]Chang C, Yang M, Wen H, Chern J: Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 2002, 10:178-182.
• [21]Velioglu YS, Mazza G, Gao L, Oomah BD: Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J Agri Food Chem 1998, 46:4113-4117.
• [22]Zu Y, Li C, Fu Y, Zhao C: Simultaneous determination of catechin, rutin, quercetin kaempferol and isorhamnetin in the extract of sea buckthorn (Hippophae rhamnoides L.) leaves by RP-HPLC with DAD. J Pharmaceut Biomed Anal 2006, 41:714-719.
• [23]Kirca A, Arslan E: Antioxidant capacity and total phenolic content of selected plants from Turkey. Intern J Food Sci Technol 2008, 43:2038-2046.
• [24]Meziti A, Meziti H, Boudiaf K, Mustapha B, Bouriche H: Polyphenolic profile and antioxidant activities of Nigella Sativa seed extracts in vitro and in vivo. World Acad Sci Eng Technol 2012, 64:24-32.
• [25]Zengin G, Aktumsek A, Guler GO, Cakmak YS, Yildiztugay E: Antioxidant properties of methanolic extract and fatty acid composition of Centaurea urvillei DC. Hayekiana Wagenitz. J Nat Prod 2011, 5:123-132.
• [26]Shah KA, Patel MD, Parmar PK, Patel RJ: In-vitro evaluation of antioxidant activity of Gymnema Sylvestre. Deccan J Nat Prod 2010, 1:1-7.
• [27]Hazra B, Biswas S, Mandal N: Antioxidant and free radical scavenging activity of Spondias pinnata. BMC Complement Altern Med 2008, 8:1-10. BioMed Central Full Text
• [28]Hachelaf A, Zellagui A, Touil A, Rhouati S (2013) Chemical composition and analysis antifungal properties of Ranunculus arvensis L. Pharmacophore 4(3):89–91. ISSN 2229-5402 USA CODEN: PHARM7
• [29]Hussain I, Ullah R, Ullah R, Khurram M, Ullah N, Baseer A, et al.: Phytochemical analysis of selected medicinal plants. Afr J Biotechnol 2011, 10:7487-7492.
• [30]Kaya GI, Somer NU, Konyalioglu S, Yalcin HT, Yavasoglu NUK, Sarikaya B, et al.: Antioxidant and antibacterial activities of Ranunculus marginatus var. trachycarpus and R. sprunerianus. Turk. J Biol 2010, 34:139-146.
• [31]Campos MG, Webby RF, Markham KR, Mitchell KA, Dacunha AP: Age-induced diminution of free radical scavenging capacity in bee pollens and the contribution of constituent flavonoids. J Agri Food Chem 2003, 51:742-745.
• [32]Prieto JM, Recio MC, Giner RM, Schinella GR, Manez S, Rios JL: In-vitro and in vivo effects of Ranunculus peltatus subsp. baudotii methanol extract on models of eicosanoid production and contact dermatitis. Phytother Res 2008, 22:297-302.
• [33]Noor W, Gul R, Ali I, Choudary MI: Isolation and antibacterial activity of the compound from Ranunculus repens L. J Chem Soc Pak 2006, 28:271-274.