Endophytic fungal association via gibberellins and indole acetic acid can improve plant growth under abiotic stress: an example of Paecilomyces formosus LHL10

BMC Microbiology

In-Jung Lee²  Joong-Hwan Lee⁴  Hee-Young Jung³  Yoon-Ha Kim²  Sang-Mo Kang²  Muhammad Hamayun¹  Abdul Latif Khan⁵ 

[1] Department of Botany, Abdul Wali Khan University, Mardan Pakistan;School of Applied Biosciences, Kyungpook National University, Daegu, Republic of Korea;Division of Applied Biology and Chemistry, College of Agriculture and Life Sciences, Kyungpook National University, Daegu, Republic of Korea;Gyeongsangbuk- Do Agricultural Research and Extension Services, Division of Agriculture Environment Research, Daegu, Republic of Korea;Department of Plant Sciences, Kohat University of Science & Technology, Kohat Pakistan

关键词: Endogenous plant hormones;    Gibberellins and indole acetic acid;    Cucumber plant growth;    Salinity;    Paecilomyces formosus LHL10;   

Others  :  1222051
DOI  :  10.1186/1471-2180-12-3

 received in 2011-07-29, accepted in 2012-01-12,  发布年份 2012

【 摘 要 】

Background

Endophytic fungi are little known for exogenous secretion of phytohormones and mitigation of salinity stress, which is a major limiting factor for agriculture production worldwide. Current study was designed to isolate phytohormone producing endophytic fungus from the roots of cucumber plant and identify its role in plant growth and stress tolerance under saline conditions.

Results

We isolated nine endophytic fungi from the roots of cucumber plant and screened their culture filtrates (CF) on gibberellins (GAs) deficient mutant rice cultivar Waito-C and normal GAs biosynthesis rice cultivar Dongjin-byeo. The CF of a fungal isolate CSH-6H significantly increased the growth of Waito-C and Dongjin-byeo seedlings as compared to control. Analysis of the CF showed presence of GAs (GA₁, GA₃, GA₄, GA₈, GA₉, GA₁₂, GA₂₀ and GA₂₄) and indole acetic acid. The endophyte CSH-6H was identified as a strain of Paecilomyces formosus LHL10 on the basis of phylogenetic analysis of ITS sequence similarity. Under salinity stress, P. formosus inoculation significantly enhanced cucumber shoot length and allied growth characteristics as compared to non-inoculated control plants. The hypha of P. formosus was also observed in the cortical and pericycle regions of the host-plant roots and was successfully re-isolated using PCR techniques. P. formosus association counteracted the adverse effects of salinity by accumulating proline and antioxidants and maintaining plant water potential. Thus the electrolytic leakage and membrane damage to the cucumber plants was reduced in the association of endophyte. Reduced content of stress responsive abscisic acid suggest lesser stress convened to endophyte-associated plants. On contrary, elevated endogenous GAs (GA₃, GA₄, GA₁₂ and GA₂₀) contents in endophyte-associated cucumber plants evidenced salinity stress modulation.

Conclusion

The results reveal that mutualistic interactions of phytohormones secreting endophytic fungi can ameliorate host plant growth and alleviate adverse effects of salt stress. Such fungal strain could be used for further field trials to improve agricultural productivity under saline conditions.

【 授权许可】

   
2012 Khan et al; licensee BioMed Central Ltd.

【 图 表 】

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