Identification of a distinct class of cytoskeleton-associated mRNAs using microarray technology

BMC Cell Biology

Donald E Ingber¹  Sui Huang¹  Amy Brock¹ 

[1] Vascular Biology Program, Departments of Pathology and Surgery, Harvard Medical School and Children's Hospital, Enders 1007, 300 Longwood Ave, Boston, MA 02115, USA

关键词: subcellular localization;    post-transcriptional control;    cytoskeleton;    actin;    gene profiling;    gene expression;    mRNA localization;   

Others  :  1088933
DOI  :  10.1186/1471-2121-4-6

 received in 2003-02-23, accepted in 2003-07-08,  发布年份 2003

【 摘 要 】

Background

Interactions between mRNA and the cytoskeleton are critical for the localization of a number of transcripts in eukaryotic somatic cells. To characterize additional transcripts that may be subject to this form of regulation, we developed a two-step approach that utilizes biochemical fractionation of cells to isolate transcripts from different subcellular compartments followed by microarray analysis to examine and compare these subpopulations of transcripts in a massively-parallel manner.

Results

Using this approach, mRNA was extracted from the cytoskeleton-rich and the cytosolic fractions of the promyelocytic HL-60 cell line. We identify a subset of 22 transcripts that are significantly enriched in the cytoskeleton-associated population. The majority of these encode structural proteins and/or proteins known to interact with elements of the cytoskeleton. Localization required an intact actin cytoskeleton and was largely conserved upon differentiation of precursor HL-60 cells to a macrophage-like phenotype.

Conclusions

We conclude that the association of transcripts with the actin cytoskeleton in somatic cells may be a critical post-transcriptional regulatory event that controls a larger class of genes than has previously been recognized.

【 授权许可】

   
2003 Brock et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.

【 图 表 】

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