Microarray analysis reveals marked intestinal microbiota aberrancy in infants having eczema compared to healthy children in at-risk for atopic disease

BMC Microbiology

Willem M de Vos⁵  Seppo Salminen⁶  Erika Isolauri²  Marko Kalliomäki²  Mirjana Rajilić-Stojanović³  Janne Nikkilä⁴  Reetta Satokari¹  Lotta Nylund¹ 

[1] Department of Veterinary Biosciences and Department of Bacteriology and Immunology, University of Helsinki, Helsinki, Finland;Department of Pediatrics, University of Turku and Turku University Hospital, Turku, Finland;Department for Biotechnology and Biochemical Engineering, Faculty of Technology and Metallurgy, University of Belgrade, Belgrade, Serbia;Finnish Red Cross Blood Service, Helsinki, Finland;Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands;Functional Foods Forum, University of Turku, Turku, FI-20014, Finland

关键词: Eczema;    Phylogenetic microarray;    Microbiota diversity;    Intestinal microbiota;    Infant;   

Others  :  1144552
DOI  :  10.1186/1471-2180-13-12

 received in 2012-06-20, accepted in 2012-11-26,  发布年份 2013

【 摘 要 】

Background

Deviations in composition and diversity of intestinal microbiota in infancy have been associated with both the development and recurrence of atopic eczema. Thus, we decided to use a deep and global microarray-based method to characterize the diversity and temporal changes of the intestinal microbiota in infancy and to define specific bacterial signatures associated with eczema. Faecal microbiota at 6 and 18 months of age were analysed from 34 infants (15 with eczema and 19 healthy controls) selected from a prospective follow-up study based on the availability of faecal samples. The infants were originally randomized to receive either Lactobacillus rhamnosus GG or placebo.

Results

Children with eczema harboured a more diverse total microbiota than control subjects as assessed by the Simpson’s reciprocal diversity index of the microarray profiles. Composition of the microbiota did not differ between study groups at age of 6 months, but was significantly different at age of 18 months as assessed by MCPP (p=0.01). At this age healthy children harboured 3 -fold greater amount of members of the Bacteroidetes (p=0.01). Microbiota of children suffering from eczema had increased abundance of the Clostridium clusters IV and XIVa, which are typically abundant in adults. Probiotic Lactobacillus rhamnosus GG supplementation in early infancy was observed to have minor long-term effects on the microbiota composition.

Conclusion

A diverse and adult-type microbiota in early childhood is associated with eczema and it may contribute to the perpetuation of eczema.

【 授权许可】

   
2013 Nylund et al.; licensee BioMed Central Ltd.

【 图 表 】

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