Influenza B virus has global ordered RNA structure in (+) and (−) strands but relatively less stable predicted RNA folding free energy than allowed by the encoded protein sequence

BMC Research Notes

Douglas H Turner¹  Walter N Moss¹  Salvatore F Priore¹ 

[1]Department of Chemistry and Center for RNA Biology, University of Rochester, Rochester, NY 14627-0216, USA

关键词: GORS;    Structural bioinformatics;    Influenza B;    Influenza A;    Influenza;    RNA secondary structure;    RNA;   

Others  :  1141851
DOI  :  10.1186/1756-0500-6-330

 received in 2013-01-15, accepted in 2013-07-03,  发布年份 2013

【 摘 要 】

Background

Influenza A virus contributes to seasonal epidemics and pandemics and contains Global Ordered RNA structure (GORS) in the nucleoprotein (NP), non-structural (NS), PB2, and M segments. A related virus, influenza B, is also a major annual public health threat, but unlike influenza A is very selective to human hosts. This study extends the search for GORS to influenza B.

Findings

A survey of all available influenza B sequences reveals GORS in the (+) and (−)RNAs of the NP, NS, PB2, and PB1 gene segments. The results are similar to influenza A, except GORS is observed for the M1 segment of influenza A but not for PB1. In general, the folding free energies of human-specific influenza B RNA segments are less stable than allowable by the encoded amino acid sequence. This is consistent with findings in influenza A, where human-specific influenza RNA folds are less stable than avian and swine strains.

Conclusions

These results reveal fundamental molecular similarities and differences between Influenza A and B and suggest a rational basis for choosing segments to target with therapeutics and for viral attenuation for live vaccines by altering RNA folding stability.

【 授权许可】

   
2013 Priore et al.; licensee BioMed Central Ltd.

【 图 表 】

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