Community-level assessment of dental plaque bacteria susceptibility to triclosan over 19 years

BMC Oral Health

Joseph J Zambon³  Prem K Sreenivasan²  Violet I Haraszthy¹ 

[1] School of Dental Medicine, University at Buffalo, Squire Hall, 3435 Main Street, Buffalo NY 14214-3008, USA;Colgate-Palmolive Technology Center, 909 River Road, Piscataway NJ 08855, USA;University at Buffalo, School of Dental Medicine, 222 Foster Hall, 3435 Main Street, Buffalo NY 14214-3008, USA

关键词: Dental plaque;    Antimicrobial resistance;    Toothpaste;    Bacteria;    Antimicrobial susceptibility;    Triclosan;   

Others  :  1109667
DOI  :  10.1186/1472-6831-14-61

 received in 2013-12-11, accepted in 2014-05-27,  发布年份 2014

【 摘 要 】

Background

Triclosan is a broad-spectrum antimicrobial agent used in toothpaste to reduce dental plaque, gingivitis and oral malodor. This community-level assessment evaluated the susceptibility of dental plaque bacteria to triclosan in samples collected over 19 years.

Methods

A total of 155 dental plaque samples were collected at eleven different times over 19 years from 58 adults using 0.3% triclosan, 2% copolymer, 0.243% sodium fluoride toothpaste and from 97 adults using toothpaste without triclosan. These included samples from 21 subjects who used triclosan toothpaste for at least five years and samples from 20 control subjects. The samples were cultured on media containing 0, 7.5 or 25 μg/ml triclosan. Descriptive statistics and p values were computed and a linear regression model and the runs test were used to examine susceptibility over time.

Results

Growth inhibition averaged 99.451% (91.209 - 99.830%) on media containing 7.5 μg/ml triclosan and 99.989% (99.670 - 100%) on media containing 25 μg/ml triclosan. There was no change in microbial susceptibility to triclosan over time discernible by regression analysis or the runs test in plaque samples taken over 19 years including samples from subjects using a triclosan-containing dentifrice for at least five years.

Conclusions

This community-level assessment of microbial susceptibility to triclosan among supragingival plaque bacteria is consistent with the long-term safety of a 0.3% triclosan, 2% copolymer, 0.243% sodium fluoride dentifrice.

【 授权许可】

   
2014 Haraszthy et al.; licensee BioMed Central Ltd.

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