Comparative genome analysis identifies few traits unique to the Escherichia coli ST131 H30Rx clade and extensive mosaicism at the capsule locus

BMC Genomics

Alan McNally¹  Zhiyong Zong²  Fleming Scheutz³  Abdulaziz Alqasim¹ 

[1] Pathogen Research Group, Nottingham Trent University, Nottingham NG11 8NS, UK;Department of Infection Control, West China Medical School, Chengdu, China;Department of Microbiolgy and Infection, Statens Serum Institute, Copenhagen, Denmark

关键词: Recombination;    Capsule;    Comparative genomics;    E. coli ST131;   

Others  :  1139481
DOI  :  10.1186/1471-2164-15-830

 received in 2014-06-18, accepted in 2014-09-24,  发布年份 2014

【 摘 要 】

Background

E.coli ST131 is a globally disseminated clone of multi-drug resistant E. coli responsible for that vast majority of global extra-intestinal E. coli infections. Recent global genomic epidemiological studies have highlighted the highly clonal nature of this group of bacteria, however there appears to be inconsistency in some phenotypes associated with the clone, in particular capsule types as determined by K-antigen testing both biochemically and by PCR.

Results

We performed improved quality assemblies on ten ST131 genomes previously sequenced by our group and compared them to a new reference genome sequence JJ1886 to identify the capsule loci across the drug-resistant clone H30Rx. Our data shows considerable genetic diversity within the capsule locus of H30Rx clone strains which is mirrored by classical K antigen testing. The varying capsule locus types appear to be randomly distributed across the H30Rx phylogeny suggesting multiple recombination events at this locus, but that this capsule heterogeneity has little to no effect on virulence associated phenotypes in vitro.

Conclusions

Our data provides a framework for determining the capsular genetics of E. coli ST131 and further beyond to ExPEC strains, and highlights how capsular mosaicism may be an important strategy in becoming a successful globally disseminated human pathogen.

【 授权许可】

   
2014 Alqasim et al.; licensee BioMed Central Ltd.

【 图 表 】

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