Med14 cooperates with brg1 in the differentiation of skeletogenic neural crest

BMC Developmental Biology

Ian C. Scott¹  Jeffrey T. A. Burrows²  Xin Lou¹ 

[1] Heart & Stroke/Richard Lewar Centre of Excellence, University of Toronto, 101 College Street, Toronto M5G 1L7, Ontario, Canada;Department of Molecular Genetics, University of Toronto, 1 King‚s College Circle, Toronto M5S 1A8, Ontario, Canada

关键词: Jaw development;    Med14;    Craniofacial defect;    Brg1;    BAF complex;    Mediator complex;    Neural crest;   

Others  :  1234328
DOI  :  10.1186/s12861-015-0090-9

 received in 2015-04-14, accepted in 2015-10-25,  发布年份 2015

【 摘 要 】

Background

An intricate gene regulatory network drives neural crest migration and differentiation. How epigenetic regulators contribute to this process is just starting to be understood.

Results

We found that mutation of med14 or brg1 in zebrafish embryos resulted in a cluster of neural crest cell-related defects. In med14 or brg1 mutants, neural crest cells that form the jaw skeleton were specified normally and migrated to target sites. However, defects in their subsequent terminal differentiation were evident. Transplantation experiments demonstrated that med14 and brg1 are required directly in neural crest cells. Analysis of med14; brg1 double mutant embryos suggested the existence of a strong genetic interaction between members of the Mediator and BAF complexes.

Conclusions

These results suggest a critical role for Mediator and BAF complex function in neural crest development, and may also clarify the nature of defects in some craniofacial abnormalities.

【 授权许可】

   
2015 Lou et al.

【 图 表 】

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