期刊论文详细信息
BMC Research Notes
Gut microeukaryotes during anorexia nervosa: a case report
Michel Drancourt1  Didier Raoult1  Nina Gouba1 
[1] Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, Faculté de Médecine, 27, Boulevard Jean Moulin, Marseille cedex 5, France
关键词: Culture;    Polymerase chain reaction;    Microeukaryotes;    Gut;    Anorexia nervosa;   
Others  :  1134846
DOI  :  10.1186/1756-0500-7-33
 received in 2013-09-06, accepted in 2013-12-20,  发布年份 2014
PDF
【 摘 要 】

Background

Few studies have focused on eukaryote community in the human gut. Here, the diversity of microeukaryotes in the gut microbiota of an anorexic patient was investigated using molecular and culture approaches.

Case presentation

A 21-year-old Caucasian woman was admitted in an intensive care unit for severe malnutrition in anorexia nervosa. One stool specimen was collected from the anorexic patient, culture and polymerase chain reaction-based explorations yielded a restricted diversity of fungi but four microeukaryotes Tetratrichomonas sp., Aspergillus ruber, Penicillium solitum and Cladosporium bruhnei previously undescribed in the human gut.

Conclusions

Establishing microeukaryote repertoire in gut microbiota contributes to the understanding of its role in human health.

【 授权许可】

   
2014 Gouba et al.; licensee BioMed Central Ltd.

【 预 览 】
附件列表
Files Size Format View
20150306080905881.pdf 149KB PDF download
【 参考文献 】
  • [1]Scanlan PD, Marchesi JR: Micro-eukaryotic diversity of the human distal gut microbiota: qualitative assessment using culture-dependent and -independent analysis of faeces. ISME J 2008, 2:1183-1193.
  • [2]Gouba N, Raoult D, Drancourt M: Plant and fungal diversity in gut microbiota as revealed by molecular and culture investigations. PLoS One 2013, 8:e59474.
  • [3]De Gruijter JM, Van LL, Gasser RB, Verweij JJ, Brienen EA, Ziem JB, Yelifari L, Polderman AM: Polymerase chain reaction-based differential diagnosis of Ancylostoma duodenale and Necator americanus infections in humans in northern Ghana. Trop Med Int Health 2005, 10:574-580.
  • [4]Scicluna SM, Tawari B, Clark CG: DNA barcoding of Blastocystis. Protist 2006, 157:77-85.
  • [5]Duval L, Robert V, Csorba G, Hassanin A, Randrianarivelojosia M, Walston J, Nhim T, Goodman SM, Ariey F: Multiple host-switching of Haemosporidia parasites in bats. Malar J 2007, 6:157-165. BioMed Central Full Text
  • [6]Bradley BJ, Stiller M, Doran-Sheehy DM, Harris T, Chapman CA, Vigilant L, Poinar H: Plant DNA sequences from feces: potential means for assessing diets of wild primates. Am J Primatol 2007, 69:699-705.
  • [7]Pons J: DNA-based identification of preys from non-destructive, total DNA extractions of predators using arthropod universal primers. Mol Ecol Notes 2006, 6:623-626.
  • [8]Zeale MR, Butlin RK, Barker GL, Lees DC, Jones G: Taxon-specific PCR for DNA barcoding arthropod prey in bat faeces. Mol Ecol Resour 2011, 11:236-244.
  • [9]Spanakos G, Piperaki ET, Menounos PG, Tegos N, Flemetakis A, Vakalis NC: Detection and species identification of old world Leishmania in clinical samples using a PCR-based method. Trans R Soc Trop Med Hyg 2008, 102:46-53.
  • [10]Armougom F, Henry M, Vialettes B, Raccah D, Raoult D: Monitoring bacterial community of human gut microbiota reveals an increase in Lactobacillus in obese patients and methanogens in anorexic patients. PLoS One 2009, 4(e7125):491-501.
  文献评价指标  
  下载次数:27次 浏览次数:10次