Characterization of the RNase R association with ribosomes

BMC Microbiology

Cecilia M Arraiano²  Cátia Bárria²  Michal Malecki¹ 

[1] Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, Warsaw, Poland;Instituto de Tecnologia Química e Biológica (ITQB), Oeiras, Portugal

关键词: RNase R;    Ribosomes;    RNA;    Ribonucleases;   

Others  :  1141918
DOI  :  10.1186/1471-2180-14-34

 received in 2013-08-13, accepted in 2014-02-03,  发布年份 2014

【 摘 要 】

Background

In this study we employed the TAP tag purification method coupled with mass spectrometry analysis to identify proteins that co-purify with Escherichia coli RNase R during exponential growth and after temperature downshift.

Results

Our initial results suggested that RNase R can interact with bacterial ribosomes. We subsequently confirmed this result using sucrose gradient ribosome profiling joined with western blot analysis. We found that RNase R co-migrates with the single 30S ribosomal subunits. Independent data involving RNase R in the rRNA quality control process allowed us to hypothesize that the RNase R connection with ribosomes has an important physiological role.

Conclusions

This study leads us to conclude that RNase R can interact with ribosomal proteins and that this interaction may be a result of this enzyme involvement in the ribosome quality control.

【 授权许可】

   
2014 Malecki et al.; licensee BioMed Central Ltd.

【 图 表 】

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