Effects of mining chemicals on fish: exposure to tailings containing Lilaflot D817M induces CYP1A transcription in Atlantic salmon smolt

BMC Research Notes

Torstein Kristensen²  John B. Ulvund³  Tom O. Nilsen⁴  Henning A. Urke⁵  Pål A. Olsvik¹ 

[1] National Institute of Nutrition and Seafood Research, Nordnesboder 1-2, Bergen, 5005, Norway;Faculty of Biosciences and Aquaculture, University of Nordland, Bodø, 8049, Norway;Norwegian Institute of Water Research, Trondheim, 7486, Norway;UNI Research, Bergen, 5006, Norway;INAQ AS, Trondheim, 7462, Norway

关键词: Transcriptional responses;    Lilaflot D817M;    Flotation chemicals;    Atlantic salmon;    Mining activity;   

Others  :  1230437
DOI  :  10.1186/s13104-015-1342-2

 received in 2014-08-08, accepted in 2015-08-12,  发布年份 2015

【 摘 要 】

Background

Mine tailings, containing metals and production chemicals such as flotation chemicals and flocculants, may pose an environmental threat to aquatic organisms living in downstream ecosystems. The aim of this work was to study to which degree Lilaflot D817M, a flotation chemical extensively used by the mining industry, represents a hazard for migrating salmon in rivers affected by mining activity. Smoltifying Atlantic salmon were exposed to four concentrations of iron-ore mine tailings containing residual Lilaflot D817M [water versus tailing volumes of 0.002 (Low), 0.004 (Medium), 0.013 (High) and 0.04 (Max)]. After 96 h of exposure, gill and liver tissues were harvested for transcriptional responses. Target genes included markers for oxidative stress, detoxification, apoptosis and DNA repair, cell signaling and growth.

Results

Of the 16 evaluated markers, significant transcriptional responses of exposure to tailings enriched with Lilaflot D817M were observed for CYP1A, HSP70 and HMOX1 in liver tissue and CYP1A in gill tissue. The significant induction of CYP1A in both liver and gills suggest that the flotation chemical is taken up by the fish and activates cytochrome P450 detoxification via phase I biotransformation in the cells.

Conclusions

The overall weak transcriptional responses to short-term exposure to Lilaflot D817M-containing iron-ore tailings suggest that the mining chemical has relatively low toxic effect on fish. The underlying mechanisms behind the observed CYP1A induction should be studied further.

【 授权许可】

   
2015 Olsvik et al.

【 图 表 】

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