Placenta-specific novel splice variants of Rho GDP dissociation inhibitor β are highly expressed in cancerous cells

BMC Research Notes

Tohru Mochizuki⁴  Ken Yamaguchi²  Masanori Terashima¹  Keiichi Ohshima⁴  Yorikane Fukuda³  Keiichi Hatakeyama⁴ 

[1] Gastric Surgery Division, Shizuoka Cancer Center Hospital, 1007 Shimonagakubo, Nagaizumi-cho, Sunto-gun, Shizuoka, 411-8777, Japan;Shizuoka Cancer Center Hospital and Research Institute, 1007 Shimonagakubo, Nagaizumi-cho, Sunto-gun, Shizuoka, 411-8777, Japan;Present address: G&G Science, 4-1-1 Misato, Matsukawa-machi, Fukusima, 960-1242, Japan;Medical Genetics Division, Shizuoka Cancer Center Research Institute, 1007 Shimonagakubo, Nagaizumi-cho, Sunto-gun, Shizuoka, 411-8777, Japan

关键词: Splice variant;    Metastasis;    Biomarker;    ARHGDIB;    Alternative splicing;   

Others  :  1165081
DOI  :  10.1186/1756-0500-5-666

 received in 2012-09-28, accepted in 2012-11-30,  发布年份 2012

【 摘 要 】

Background

Alternative splicing of pre-mRNA transcripts not only plays a role in normal molecular processes but is also associated with cancer development. While normal transcripts are ubiquitously expressed in normal tissues, splice variants created through abnormal alternative splicing events are often expressed in cancer cells. Although the Rho GDP dissociation inhibitor β (ARHGDIB) gene has been found to be ubiquitously expressed in normal tissues and involved in cancer development, the presence of splice variants of ARHGDIB has not yet been investigated.

Results

Validation analysis for the presence of and exon structures of splice variants of ARHGDIB, performed using reverse-transcriptase polymerase chain reaction and DNA sequencing, successfully identified novel splice variants of ARHGDIB, that is, 6a, 6b, and 6c, in colon, pancreas, stomach, and breast cancer cell lines. Quantitative real-time polymerase chain reaction analysis showed that these variants were also highly expressed in normal placental tissue but not in other types of normal tissue.

Conclusions

Expression of ARHGDIB variants 6a, 6b, and 6c appears to be restricted to cancer cells and normal placental tissue, suggesting that these variants possess cancer-specific functions and, as such, are potential cancer-related biomarkers.

【 授权许可】

   
2012 Hatakeyama et al.; licensee BioMed Central Ltd.

【 图 表 】

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