Characterising the aetiology of severe acute gastroenteritis among patients visiting a hospital in Qatar using real-time polymerase chain reaction

BMC Infectious Diseases

Said Al-Dhahry²  Najah Al-Lawati¹  Maide Baris¹  Asma Al-Thani¹ 

[1]Qatar University, P.O. BOX 2713, Doha, Qatar

[2]Laboratory Medicine Department, Hamad Medical Corporation, Doha, Qatar

关键词: Acute gastroenteritis;    Astrovirus;    Adenovirus;    Rotavirus;    Norovirus;   

Others  :  1146930
DOI  :  10.1186/1471-2334-13-329

 received in 2013-01-09, accepted in 2013-07-05,  发布年份 2013

【 摘 要 】

Background

Acute gastroenteritis (AGE) remains a common cause of clinic visits and hospitalizations, though its aetiology has not been determined in Qatar.

Methods

We performed a prospective, emergency department–based study of 288 children and adults with AGE. Stool specimens were collected at presentation from June to November 2009. Faecal specimens were tested, using real-time PCR, for a panel of four viral (norovirus, adenovirus, astrovirus and rotavirus) and bacterial pathogens.

Results

Viral and bacterial pathogens were detected in 131 (45.5%) and 34 (12.2%) of the 288 patients recruited. The most commonly detected pathogens were norovirus (28.5%), rotavirus (10.4%), followed by adenovirus (6.25%) and astrovirus (0.30%). Norovirus was the most commonly detected viral pathogen amongst all the age groups with an almost even distribution in all age groups. Rotavirus and adenovirus were more common in children under 5 yr of age. Astrovirus was found in only one person.

Conclusions

Viruses, especially noroviruses, are associated with severe diarrhoea in children and adults in Qatar. Further studies to confirm the findings and to explore the causes of illness among patients from whom a pathogen cannot be determined are needed.

【 授权许可】

   
2013 Al-thani et al.; licensee BioMed Central Ltd.

【 参考文献 】

• [1]Chow CM, Leung AK, Hon KL: Acute gastroenteritis: from guidelines to real life. Clin Exp Gastroenterol 2010, 3:97-112.
• [2]Bresee JS, Marcus R, Venezia RA, Keene WE, Morse D, Thanassi M, Brunett P, Bulens S, Beard RS, Dauphin LA, Slutsker L, Bopp C, Eberhard M, Hall A, Vinje J, Monroe SS, Glass RI, US Acute Gastroenteritis Etiology Study Team: The etiology of severe acute gastroenteritis among adults visiting emergency departments in the United States. J Infect Dis 2012, 205:1374-1381.
• [3]Clark B, McKendrick M: A review of viral gastroenteritis. Curr Opin Infect Dis 2004, 17:461-469.
• [4]Wood DJ: Adenovirus gastroenteritis. Br Med J (Clin Res Ed) 1988, 296(6617):229-230.
• [5]Monroe SS: Control and prevention of viral gastroenteritis. Emerg Infect Dis 2011, 17(8):1347-1348.
• [6]Patel MM, Hall AJ, Vinje J, Parashar UD: Noroviruses: a comprehensive review. J Clin Virol 2009, 44:1-8.
• [7]Haustein T, Harris JP, Pebody R, Lopman BA: Hospital admissions due to norovirus in adult and elderly patients in England. Clin Infect Dis 2009, 49:1890-1892.
• [8]Rahman M, Hassan Z, Nahar Z, Faruque AS, Van Ranst M, Rahman SR, Azim T: Molecular detection of noroviruses in hospitalized patients in Bangladesh. Eur J Clin Microbiol Infect Dis 2010, 29:937-945.
• [9]Khoury H, Ogilvie I, El Khoury AC, Duan Y, Goetghebeur MM: Burden of rotavirus gastroenteritis in the Middle Eastern and North African pediatric population. BMC Infect Dis 2011, 11:9. BioMed Central Full Text
• [10]Parashar UD, Hummelman EG, Bresee JS, Miller MA, Glass RI: Global illness and deaths caused by rotavirus disease in children. Emerg Infect Dis 2003, 9:565-572.
• [11]Ramani S, Kang G: Viruses causing childhood diarrhoea in the developing world. Curr Opin Infect Dis 2009, 22:477-482.
• [12]Tran A, Talmud D, Lejeune B, Jovenin N, Renois F, Payan C, Leveque N, Andreoletti L: Prevalence of rotavirus, adenovirus, norovirus, and astrovirus infections and coinfections among hospitalized children in Northern France. J Clin Microbiol 2010, 48:1943-1946.
• [13]Li CY, Liu N, Guo WD, Yu Q, Wang WR, Song ZZ, Yan H, Luo Y, Lu AT, Li HY, Zhu L, Duan ZJ: Outbreak of neonatal gastroenteritis associated with astrovirus serotype 1 at a hospital in Inner Mongollia, China. J Clin Microbiol 2010, 48:4306-4309.
• [14]Amar CF, East CL, Gray J, Iturriza-Gomara M, Maclure EA, McLauchlin J: Detection by PCR of eight groups of enteric pathogens in 4,627 faecal samples: re-examination of the English case–control Infectious Intestinal Disease Study (1993–1996). Eur J Clin Microbiol Infect Dis 2007, 26:311-323.
• [15]Karsten C, Baumgarte S, Friedrich AW, von Eiff C, Becker K, Wosniok W, Ammon A, Bockemühl J, Karch H, Huppertz HI: Incidence and risk factors for community-acquired acute gastroenteritis in north-west Germany in 2004. Eur J Clin Microbiol Infect Dis 2009, 28:935-943.
• [16]Huhulescu S, Kiss R, Brettlecker M, Cerny RJ, Hess C, Wewalka G, Allerberger F: Etiology of acute gastroenteritis in three sentinel general practices, Austria 2007. Infection 2009, 37:103-108.
• [17]de Wit MA, Koopmans MP, Kortbeek LM, Wannet WJ, Vinjé J, van Leusden F, Bartelds AI, van Duynhoven YT: Sensor, a population-based cohort study on gastroenteritis in the Netherlands: incidence and etiology. Am J Epidemiol 2001, 154:666-674.
• [18]de Wit MA, Koopmans MP, Kortbeek LM, van Leeuwen NJ, Vinje J, van Duynhoven YT: Etiology of gastroenteritis in sentinel general practices in The Netherlands. Clin Infect Dis 2001, 33:280-288.
• [19]Rockx B, De Wit M, Vennema H, Vinjé J, De Bruin E, Van Duynhoven Y, Koopmans M: Natural history of human calicivirus infection: a prospective cohort study. Clin Infect Dis 2002, 35:246-253.
• [20]Wolfaardt M, Taylor MB, Booysen HF, Engelbrecht L, Grabow WO, Jiang X: Incidence of human calicivirus and rotavirus infection in patients with gastroenteritis in South Africa. J Med Virol 1997, 51:290-296.
• [21]Staat MA, Azimi PH, Berke T, Roberts N, Bernstein DI, Ward RL, Pickering LK, Matson DO: Clinical presentations of rotavirus infection among hospitalized children. Pediatr Infect Dis J 2002, 21:221-227.
• [22]de Wit MA, Koopmans MP, Kortbeek LM, van Leeuwen NJ, Bartelds AI, van Duynhoven YT: Gastroenteritis in sentinel general practices, The Netherlands. Emerg Infect Dis 2001, 7:82-91.
• [23]Cortese MM, Tate JE, Simonsen L, Edelman L, Parashar UD: Reduction in gastroenteritis in United States children and correlation with early rotavirus vaccine uptake from national medical claims databases. Pediatr Infect Dis J 2010, 29:489-494.