Retrospective observational study of diagnostic accuracy of the Xpert® MTB/RIF assay on fiberoptic bronchoscopy sampling for early diagnosis of smear-negative or sputum-scarce patients with suspected tuberculosis

BMC Pulmonary Medicine

Emmanuel Bergot¹  Gérard Zalcman²  Youssef Oulkhouir³  Karine Campbell²  Romain Magnier³  Brigitte Malbruny⁴  Vincent Cattoir²  Pierre Le Palud³ 

[1] Groupe pour la Recherche et l’Enseignement en Pneumo-Infectiologie (GREPI) de la Société de Pneumologie de Langue Française (SPLF), Paris, F-75000, France;Université de Caen-Basse Normandie, Caen, F-14000, France;CHU de Caen, Service de Pneumologie et Oncologie thoracique, Caen, F-14000, France;CHU de Caen, Service de Microbiologie, Caen, F-14000, France

关键词: Smear microscopy;    Xpert® MTB/RIF assay;    Fiberoptic bronchoscopy;    Tuberculosis diagnosis;   

Others  :  1109548
DOI  :  10.1186/1471-2466-14-137

 received in 2014-03-07, accepted in 2014-07-01,  发布年份 2014

【 摘 要 】

Background

Fiberoptic bronchoscopy (FOB) is a useful diagnosis tool in low-burden countries for patients with suspected pulmonary tuberculosis (TB) who are smear-negative or sputum-scarce. This study sought to determine the accuracy of the Xpert® MTB/RIF (XP) assay using FOB samples.

Methods

We retrospectively reviewed clinical, radiological, and microbiological characteristics of 175 TB-suspected patients requiring diagnostic FOB (bronchial aspirate or bronchoalveolar lavage) with XP assay. Polymerase chain reaction (PCR) and smear microscopy (SM) performances were first compared to culture, then to the final diagnosis, established based on clinical or radiological evolution when cultures were negative.

Results

Of the total 162 included patients, 30 (18.5%) had a final diagnosis of pulmonary TB, with positive cultures reported in 23. As compared to culture, sensitivity and specificity values were 80.0% and 98.6% for the XP assay, and 25.0% and 95.8% for SM, respectively. As compared to final diagnosis, the corresponding performance values were 60.0% and 100.0% for the XP assay, and 16.7% and 95.5% for SM, respectively. The sensitivity of the XP assay was significantly higher than that of SM in both cases (p = 0.003 and p = 0.001). Concerning the final diagnosis, both XP assay and culture sensitivities were similar (60% vs. 66.7%). PCR assay enabled pulmonary TB to be diagnosed earlier in 13 more cases, compared to SM.

Conclusion

Our study has confirmed the clinical benefits provided by XP assay compared to SM for the early diagnosis of suspected pulmonary TB cases requiring FOB, on per procedure samples, especially in a low TB-burden country.

【 授权许可】

   
2014 Le Palud et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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