Efficacy of citric acid denture cleanser on the Candida albicans biofilm formed on poly(methyl methacrylate): effects on residual biofilm and recolonization process

BMC Oral Health

Altair Antoninha Del Bel Cury¹  Wander José da Silva¹  Luciana de Rezende Pinto²  Martinna de Mendonça e Bertolini¹  Yuri Wanderley Cavalcanti¹  Fernanda Faot² 

[1] Department of Prosthodontics and Periodontology, Piracicaba Dental School, State University of Campinas, Piracicaba, São Paulo 13414-903, Brazil;Department of Prosthodontics, School of Dentistry, Federal University of Pelotas, Pelotas, Rio Grande, do Sul 96015-560, Brazil

关键词: Poly(methyl methacrylate);    Candida albicans;    Denture Cleansers;    Denture hygiene;    Biofilm;   

Others  :  791692
DOI  :  10.1186/1472-6831-14-77

 received in 2014-04-04, accepted in 2014-06-20,  发布年份 2014

【 摘 要 】

Background

It is well known that the use of denture cleansers can reduce Candida albicans biofilm accumulation; however, the efficacy of citric acid denture cleansers is uncertain. In addition, the long-term efficacy of this denture cleanser is not well established, and their effect on residual biofilms is unknown. This in vitro study evaluated the efficacy of citric acid denture cleanser treatment on C. albicans biofilm recolonization on poly(methyl methacrylate) (PMMA) surface.

Methods

C. albicans biofilms were developed for 72 h on PMMA resin specimens (n = 168), which were randomly assigned to 1 of 3 cleansing treatments (CTs) overnight (8 h). CTs included purified water as a control (CTC) and two experimental groups that used either a 1:5 dilution of citric acid denture cleanser (CT5) or a 1:8 dilution of citric acid denture cleanser (CT8). Residual biofilms adhering to the specimens were collected and quantified at two time points: immediately after CTs (ICT) and after cleaning and residual biofilm recolonization (RT). Residual biofilms were analyzed by quantifying the viable cells (CFU/mL), and biofilm architecture was evaluated by confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). Denture cleanser treatments and evaluation periods were considered study factors. Data were analyzed using two-way ANOVA and Tukey’s Honestly Significant Difference (HSD) test (α = 0.05).

Results

Immediately after treatments, citric acid denture cleansing solutions (CT5 and CT8) reduced the number of viable cells as compared with the control (p < 0.01). However, after 48 h, both CT groups (CT5 and CT8) showed biofilm recolonization (p < 0.01). Residual biofilm recolonization was also detected by CLSM and SEM analysis, which revealed a higher biomass and average biofilm thickness for the CT8 group (p < 0.01).

Conclusion

Citric acid denture cleansers can reduce C. albicans biofilm accumulation and cell viability. However, this CT did not prevent biofilm recolonization.

【 授权许可】

   
2014 Faot et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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