Production, purification, sequencing and activity spectra of mutacins D-123.1 and F-59.1

BMC Microbiology

Marc C Lavoie²  Gisèle LaPointe¹  Guillaume G Nicolas¹ 

[1]Centre de Recherche en Sciences et Technologie du Lait (STELA), Institut des Nutraceutiques et des Aliments Fonctionnels (INAF), Faculté des Sciences de l'Agriculture et de l'Alimentation, Université Laval, Québec (Québec), G1V 0A6, Canada

[2]Department of Biological and Chemical Sciences, Faculty of Pure and Applied Sciences, The University of the West Indies, Cave Hill Campus, P.O. Box 64 Bridgetown, BB11000, Barbados

关键词: Streptococcus mutans;    pediocin;    mutacin;    lantibiotic;    bacteriocin;   

Others  :  1225268
DOI  :  10.1186/1471-2180-11-69

 received in 2010-09-29, accepted in 2011-04-10,  发布年份 2011

【 摘 要 】

Background

The increase in bacterial resistance to antibiotics impels the development of new anti-bacterial substances. Mutacins (bacteriocins) are small antibacterial peptides produced by Streptococcus mutans showing activity against bacterial pathogens. The objective of the study was to produce and characterise additional mutacins in order to find new useful antibacterial substances.

Results

Mutacin F-59.1 was produced in liquid media by S. mutans 59.1 while production of mutacin D-123.1 by S. mutans 123.1 was obtained in semi-solid media. Mutacins were purified by hydrophobic chromatography. The amino acid sequences of the mutacins were obtained by Edman degradation and their molecular mass was determined by mass spectrometry. Mutacin F-59.1 consists of 25 amino acids, containing the YGNGV consensus sequence of pediocin-like bacteriocins with a molecular mass calculated at 2719 Da. Mutacin D-123.1 has an identical molecular mass (2364 Da) with the same first 9 amino acids as mutacin I. Mutacins D-123.1 and F-59.1 have wide activity spectra inhibiting human and food-borne pathogens. The lantibiotic mutacin D-123.1 possesses a broader activity spectrum than mutacin F-59.1 against the bacterial strains tested.

Conclusion

Mutacin F-59.1 is the first pediocin-like bacteriocin identified and characterised that is produced by Streptococcus mutans. Mutacin D-123.1 appears to be identical to mutacin I previously identified in different strains of S. mutans.

【 授权许可】

   
2011 Nicolas et al; licensee BioMed Central Ltd.

【 图 表 】

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