A DArT marker-based linkage map for wild potato Solanum bulbocastanum facilitates structural comparisons between Solanum A and B genomes

BMC Genetics

James M Bradeen¹  Domenico Carputo⁴  Riccardo Aversano⁴  Andrzej Kilian³  Maria Luisa Chiusano⁴  Alessandra Traini²  Harpartap Mann⁵  Liangliang Gao⁵  Massimo Iorizzo⁴ 

[1] Stakman-Borlaug Center for Sustainable Plant Health, 495 Borlaug Hall/1991 Upper Buford Circle, St. Paul MN 55108, USA;Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, London, United Kingdom;Diversity Arrays Technology, Pty. Ltd., University of Canberra, Kirinari Street, Canberra, ACT 2617, Bruce, Australia;Department of Agricultural Sciences, University of Naples Federico II, Via Università 100, Portici, 80055, Italy;Department of Plant Pathology, University of Minnesota, 495 Borlaug Hall/1991 Upper Buford Circle, St. Paul 55108, MN, USA

关键词: Comparative genomics;    S. lycopersicum;    S. tuberosum;    DArT markers;    Linkage map;    S. bulbocastanum;   

Others  :  1085298
DOI  :  10.1186/s12863-014-0123-6

 received in 2014-05-16, accepted in 2014-10-29,  发布年份 2014

【 摘 要 】

Background

Wild potato Solanum bulbocastanum is a rich source of genetic resistance against a variety of pathogens. It belongs to a taxonomic group of wild potato species sexually isolated from cultivated potato. Consistent with genetic isolation, previous studies suggested that the genome of S. bulbocastanum (B genome) is structurally distinct from that of cultivated potato (A genome). However, the genome architecture of the species remains largely uncharacterized. The current study employed Diversity Arrays Technology (DArT) to generate a linkage map for S. bulbocastanum and compare its genome architecture with those of potato and tomato.

Results

Two S. bulbocastanum parental linkage maps comprising 458 and 138 DArT markers were constructed. The integrated map comprises 401 non-redundant markers distributed across 12 linkage groups for a total length of 645 cM. Sequencing and alignment of DArT clones to reference physical maps from tomato and cultivated potato allowed direct comparison of marker orders between species. A total of nine genomic segments informative in comparative genomic studies were identified. Seven genome rearrangements correspond to previously-reported structural changes that have occurred since the speciation of tomato and potato. We also identified two S. bulbocastanum genomic regions that differ from cultivated potato, suggesting possible chromosome divergence between Solanum A and B genomes.

Conclusions

The linkage map developed here is the first medium density map of S. bulbocastanum and will assist mapping of agronomical genes and QTLs. The structural comparison with potato and tomato physical maps is the first genome wide comparison between Solanum A and B genomes and establishes a foundation for further investigation of B genome-specific structural chromosome rearrangements.

【 授权许可】

   
2014 Iorizzo et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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