Effect of Uncaria tomentosa extract on purinergic enzyme activities in lymphocytes of rats submitted to experimental adjuvant arthritis model

BMC Complementary and Alternative Medicine

Daniela B. R. Leal³  Jamile F. Gonçalves³  Margareth L. Athayde²  Juliano Ferreira³  Ritiel Corrêa da Cruz²  Emerson André Casalli¹  Mariane A. da Silva³  Mateus F. Rossato³  Pedro H. Doleski⁴  Josiane B. Schlemmer³  Jeandre A. dos S. Jaques³  Maria Luiza Thorstenberg³  Jader B. Ruchel³  João F. P. Rezer³  Lívia G. Castilhos⁴ 

[1] Laboratório de Enzimologia Aplicada ao Sistema Purinérgico, Departamento de Bioquímica, Instituto deCiências Básicas da Saúde, UFRGS, Porto Alegre, RS, Brazil;Departamento de Farmácia Industrial, Universidade Federal de Santa Maria, Av. Roraima, Santa Maria, 97105-900, RS, Brazil;Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Av. Roraima, Santa Maria, 97105-900, RS, Brazil;Departamento de Microbiologia e Parasitologia, Centro de Ciências da Saúde, Universidade Federal deSanta Maria, Av. Roraima, Prédio 20 – Sala 4102, Santa Maria, 97105-900, RS, Brazil

关键词: Adenosine deaminase;    E-NTPDase;    lymphocytes;    Uncaria tomentosa;    Adjuvant arthritis;   

Others  :  1217070
DOI  :  10.1186/s12906-015-0694-4

 received in 2014-04-14, accepted in 2015-05-27,  发布年份 2015

【 摘 要 】

Background

Considering that adjuvant arthritis is an experimental model of arthritis widely used for preclinical testing of numerous anti-arthritic agents, which were taken by a large number of patients worldwide, it is of great interest to investigate the therapeutic action of compounds with anti-inflammatory properties, such as Uncaria tomentosa extract. Moreover, there are no studies demonstrating the effect of U. tomentosa on the metabolism of adenine nucleotides published so far. Thus, the purpose of the present study is to investigate the effects of U. tomentosa extract on E-NTPDase and E-ADA activities in lymphocytes of Complete Freund’s Adjuvant (CFA) arthritis induced rats.

Methods

To evaluate the effect of U. tomentosa extract on the activity of E-NTPDase and ADA in lymphocytes, the rats were submitted to an experimental adjuvant arthritis model. Peripheral lymphocytes were isolated and E-NTPDase and E-ADA activities were determined. Data were analyzed by a one- or two-way ANOVA. Post hoc analyses were carried out by the Student-Newman-Keuls (SNK) Multiple Comparison Test.

Results

E-NTPDase activity was increased in arthritic untreated. Arthritic rats which received U. tomentosa extract, presented similar results to the control group. However, results obtained for adenosine hydrolysis by E-ADA were not altered in arthritic rats. U. tomentosa extract did not alter E-NTPDase and E-ADA activity in healthy animals.

Conclusions

The present investigation supports the hypothesis that the increased E-NTPDase activity verified in arthritic rats might be an attempt to maintain basal levels of ATP and ADP in the extracellular medium, since the arthritis induction causes tissue damage and, consequently, large amounts of ATP are released into this milieu. Also, it highlights the possibility to use U. tomentosa extract as an adjuvant to treat arthritis.

【 授权许可】

   
2015 Castilhos et al.

【 图 表 】

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