Cross-species testing and utility of microsatellite loci in Indirana frogs

BMC Research Notes

Juha Merilä¹  K Santhosh Kumar²  Sanil George²  Sujith V Gopalan²  Abhilash Nair¹ 

[1] Ecological Genetics Research Unit, Department of Biosciences, University of Helsinki, PO Box 65, Helsinki, FI-00014, Finland;Chemical Biology, Rajiv Gandhi Centre for Biotechnology, PO Thycaud, Poojappura, Thiruvananthapuram, Kerala, 695 014, India

关键词: Western Ghats;    Ranixalidae;    Biodiversity hotspot;    Indirana;    Microsatellite;    Amphibia;   

Others  :  1166056
DOI  :  10.1186/1756-0500-5-389

 received in 2012-05-16, accepted in 2012-07-13,  发布年份 2012

【 摘 要 】

Background

Microsatellite loci are widely used in population and conservation genetic studies of amphibians, but the availability of such markers for tropical and subtropical taxa is currently very limited. In order to develop resources for conservation genetic studies in the genus Indirana, we tested amplification success and polymorphism in 62 previously developed microsatellite loci, in eight Indirana species - including new candidate species. Developing genomic resources for this amphibian taxon is particularly important as it is endemic to the Western Ghats biodiversity hotspot, and harbours several endangered species.

Findings

The cross-species amplification success rate varied from 11.3 % to 29.0 % depending on the species, with 29 - 80 % of the amplifying loci being polymorphic. A strong negative correlation between cross-species amplification success (and polymorphism) and genetic distance separating target from source species was observed.

Conclusions

Our results provide additional genetic support for the existence of genetically divergent cryptic species within the genus Indirana. The tested markers should be useful for population and conservation genetic studies in this genus, and in particular, for species closely related to the source species, I. beddomii.

【 授权许可】

   
2012 Nair et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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