B Lymphocyte intestinal homing in inflammatory bowel disease

BMC Immunology

Fabiola Atzeni¹  Savino Bruno⁴  Piero Luigi Almasio⁵  Simona Bollani⁶  Simone Saibeni⁴  Annamaria Croce⁴  Silvia Grosso³  Piercarlo Sarzi-Puttini²  Caterina Defendenti³ 

[1] Experimental Medicine, Queen Mary University, London, UK;Rheumatology Unit, L. Sacco University Hospital, Milan, Italy;Laboratory Unit, Fatebenefratelli Hospital, Milan, Italy;Division of Gastroenterology, Fatebenefratelli Hospital, Milan, Italy;GI & Liver Unit, DIBIMIS, Policlinico, University of Palermo, Palermo, Italy;Division of Pathology, Fatebenefratelli Hospital, Milan, Italy

关键词: lymphocyte homing;    B1 cells;    lymphocytes;    mucosal immunity;    inflammation;    Inflammatory bowel disease;   

Others  :  1077957
DOI  :  10.1186/1471-2172-12-71

 received in 2011-07-23, accepted in 2011-12-30,  发布年份 2011

【 摘 要 】

Background

Inflammatory bowel disease (IBD) is thought to be due to an abnormal interaction between the host immune system and commensal microflora. Within the intestinal immune system, B cells produce physiologically natural antibodies but pathologically atypical anti-neutrophil antibodies (xANCAs) are frequently observed in patients with IBD. The objective is to investigate the localisation of immunoglobulin-producing cells (IPCs) in samples of inflamed intestinal tissue taken from patients with IBD, and their possible relationship with clinical features.

Methods

The IPCs in small intestinal, colonic and rectal biopsy specimens of patients with IBD were analysed by means of immunofluorescence using polyclonal rabbit anti-human Ig and goat anti-human IgM. The B cell phenotype of the IPC-positive samples was assessed using monoclonal antibodies specific for CD79, CD20, CD23, CD21, CD5, λ and κ chains. Statistical correlations were sought between the histological findings and clinical expression.

Results

The study involved 96 patients (64 with ulcerative colitis and 32 with Crohn's disease). Two different patterns of B lymphocyte infiltrates were found in the intestinal tissue: one was characterised by a strong to moderate stromal localisation of small IgM⁺/CD79⁺/CD20^-/CD21^-/CD23^-/CD5^± IPCs (42.7% of cases); in the other (57.3%) no such small IPCs were detected in stromal or epithelial tissues. IPCs were significantly less frequent in the patients with Crohn's disease than in those with ulcerative colitis (p = 0.004).

Conclusion

Our findings suggest that different immunopathogenetic pathways underlie chronic intestinal inflammation with different clinical expressions. The presence of small B lymphocytes resembling B-1 cells also seemed to be negatively associated with Crohn's disease. It can therefore be inferred that the gut contains an alternative population of B cells that have a regulatory function.

【 授权许可】

   
2011 Defendenti et al; licensee BioMed Central Ltd.

【 图 表 】

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