Detection of nitrogen deficiency QTL in juvenile wild barley introgression lines growing in a hydroponic system

BMC Genetics

Klaus Pillen¹  Andreas Maurer¹  Astrid Hoffmann¹ 

[1] Institute of Agricultural and Nutritional Sciences, Chair of Plant Breeding,Martin-Luther-University Halle-Wittenberg, Betty-Heimann-Str. 3, Halle, 06120,Germany

关键词: Juvenile plant development;    Hydroponics;    Nitrogen stress tolerance;    QTL;    Hordeum vulgare ssp. spontaneum;    Introgression lines;   

Others  :  1089688
DOI  :  10.1186/1471-2156-13-88

 received in 2012-04-30, accepted in 2012-10-11,  发布年份 2012

【 摘 要 】

Background

In this report we studied the genetic regulation of juvenile development of wild barley introgression lines (S42ILs) under two contrasting hydroponic nitrogen (N) supplies. Ten shoot and root related traits were examined among 42 S42ILs and the recurrent parent ‘Scarlett’. The traits included tiller number, leaf number, plant height, leaf and root length, leaf to root length ratio, shoots and root dry weight, shoot to root weight ratio, and chlorophyll content. Our aims were (1) to test the suitability of a hydroponic system for early detection of favourable S42ILs, (2) to locate quantitative trait loci (QTL) that control the examined traits, (3) to identify favourable wild barley alleles that improve trait performances in regard to N treatment and, finally, (4) to validate the identified QTL through comparison with previously reported QTL originating from the same parental cross.

Results

The phenotypic data were analysed in a mixed model association study to detect QTL. The post-hoc Dunnett test identified 28 S42ILs that revealed significant (P < 0.01) effects for at least one trait. Forty-three, 41 and 42 S42ILs revealed effects across both N treatments, under low N and under high N treatment, respectively. Due to overlapping or flanking wild barley introgressions of the S42ILs, these associations were summarised to 58 QTL. In total, 12 QTL of the hydroponic N study corresponded to QTL that were also detected in field trials with adult plants of a similar S42IL set or of the original S42 population. For instance, S42IL-135, -136 and -137, revealed increasing Hsp effects for tiller number, leaf number, leaf length, plant height and leaf to root ratio on the long arm of chromosome 7H. These QTL correspond to QTL for ears per plant and plant height that were previously detected in field trials conducted with the same S42ILs or with the S42 population.

Conclusion

Our results suggest that the QTL we identified under hydroponic N cultivation partly correspond to QTL detected in field experiments. Due to this finding, screening of plants in early developmental stages grown in a hydroponic system may be a fast and cost effective method for early QTL detection and marker-assisted allelic selection, potentially speeding up elite barley breeding programs.

【 授权许可】

   
2012 Hoffmann et al; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

Figure 3.

【 参考文献 】

• [1]Badr A, Muller K, Schafer-Pregl R, El Rabey H, Effgen S, Ibrahim HH, Pozzi C, Rohde W, Salamini F: On the origin and domestication history of barley (Hordeum vulgare). Mol Biol Evol 2000, 17(4):499-510.
• [2]von Bothmer R: The wild species of Hordeum: relationships and potential use for improvement of cultivated barley. In Barley: Genetics, Biochemistry, Molecular Biology, and Biotechnology. Edited by Shewry P. Wallingford: CAB Internationa; 1992:3-18.
• [3]Ellis RP, Forster BP, Robinson D, Handley LL, Gordon DC, Russell JR, Powell W: Wild barley: a source of genes for crop improvement in the 21st century? J Exp Bot 2000, 51(342):9-17.
• [4]Gur A, Zamir D: Unused natural variation can lift yield barriers in plant breeding. PLoS Biol 2004, 2(10):1610-1615.
• [5]Jorgensen JH: Discovery, characterization and exploitation of Mlo powdery mildew resistance in barley. Euphytica 1992, 63(1–2):141-152.
• [6]Tanksley SD, Nelson JC: Advanced backcross QTL analysis: a method for the simultaneous discovery and transfer of valuable QTLs from unadapted germplasm into elite breeding lines. Theor Appl Genet 1996, 92(2):191-203.
• [7]Zamir D: Improving plant breeding with exotic genetic libraries. Nat Rev Genet 2001, 2(12):983-989.
• [8]Keurentjes JJB, Bentsink L, Alonso-Blanco C, Hanhart CJ, Vries HBD, Effgen S, Vreugdenhil D, Koornneef M: Development of a near-isogenic line population of Arabidopsis thaliana and comparison of mapping power with a recombinant inbred line population. Genetics 2007, 175(2):891-905.
• [9]Szalma SJ, Hostert BM, LeDeaux JR, Stuber CW, Holland JB: QTL mapping with near-isogenic lines in maize. Theor Appl Genet 2007, 114(7):1211-1228.
• [10]Liu SB, Zhou RG, Dong YC, Li P, Jia JZ: Development, utilization of introgression lines using a synthetic wheat as donor. Theor Appl Genet 2006, 112(7):1360-1373.
• [11]Mei HW, Xu JL, Li ZK, Yu XQ, Guo LB, Wang YP, Ying CS, Luo LJ: QTLs influencing panicle size detected in two reciprocal introgressive line (IL) populations in rice (Oryza sativaL.). Theor Appl Genet 2006, 112(4):648-656.
• [12]von Korff M, Wang H, L on J, Pillen K: Development of candidate introgression lines using an exotic barley accession (Hordeum vulgare ssp. spontaneum) as donor. Theor Appl Genet 2004, 109(8):1736-1745.
• [13]Schmalenbach I, Körber N, Pillen K: Selecting a set of wild barley introgression lines and verification of QTL effects for resistance to powdery mildew and leaf rust. Theor Appl Genet 2008, 117(7):1093-1106.
• [14]Schmalenbach I, L on J, Pillen K: Identification and verification of QTLs for agronomic traits using wild barley introgression lines. Theor Appl Genet 2009, 118(3):483-497.
• [15]Sayed M, Schumann H, Pillen K, Naz A, Leon J: AB-QTL analysis reveals new alleles associated to proline accumulation and leaf wilting under drought stress conditions in barley (Hordeum vulgareL.). BMC Genet 2012, 13(1):61.
• [16]Naz AA, Ehl A, Pillen K, L on J: Validation for root-related quantitative trait locus effects of wild origin in the cultivated background of barley (Hordeum vulgareL.). Plant Breeding 2012, 3:392-398.
• [17]Schmalenbach I, Pillen K: Detection and verification of malting quality QTLs using wild barley introgression lines. Theor Appl Genet 2009, 118(8):1411-1427.
• [18]Wang G, Schmalenbach I, von Korff M, Leon J, Kilian B, Rode J, Pillen K: Association of barley photoperiod and vernalization genes with QTLs for flowering time and agronomic traits in a BC2DH population and a set of wild barley introgression lines. Theor Appl Genet 2010, 120(8):1559-1574.
• [19]Schmalenbach I, March TJ, Bringezu T, Waugh R, Pillen K: High-resolution genotyping of wild barley introgression lines and fine-mapping of the threshability locus thresh-1 using the Illumina GoldenGate assay. G3: Genes, Genomes, Genetics 2011, 1(3):187-196.
• [20]Epstein E, Bloom A: Mineral nutrition of plants 2. Principles and Perspectives. Sunderland: Sinauer; 2005.
• [21]Mickelson S, See D, Meyer FD, Garner JP, Foster CR, Blake TK, Fischer AM: Mapping of QTL associated with nitrogen storage and remobilization in barley (Hordeum vulgare L.) leaves. J Exp Bot 2003, 54(383):801-812.
• [22]Saal B, von Korff M, L on J, Pillen K: Advanced-backcross QTL analysis in spring barley: IV. Localization of QTL × nitrogen interaction effects for yield-related traits. Euphytica 2011, 177(2):223-239.
• [23]Schnaithmann F, Pillen K: Detection of exotic QTLs controlling nitrogen stress tolerance among wild barley introgression lines. Euphytica 2012.
• [24]Baethgen WE, Christianson CB, Lamothe AG: Nitrogen fertilizer effects on growth, grain yield, and yield components of malting barley. Field Crop Res 1995, 43(2–3):87-99.
• [25]Marshall B, Ellis RP: Growth, yield and grain quality of barley (Hordeum vulgare L.) in response to nitrogen uptake I. A low cost, controlled nutrient supply system. J Exp Bot 1998, 49(323):1049-1057.
• [26]Ellis RP, Marshall B: Growth, yield and grain quality of barley (Hordeum vulgare L.) in response to nitrogen uptake - II. Plant development and rate of germination. J Exp Bot 1998, 49(323):1021-1029.
• [27]Karley AJ, Valentine TA, Squire GR: Dwarf alleles differentially affect barley root traits influencing nitrogen acquisition under low nutrient supply. J Exp Bot 2011, 62(11):3917-3927.
• [28]Beatty PH, Anbessa Y, Juskiw P, Carroll RT, Wang JA, Good AG: Nitrogen use efficiencies of spring barley grown under varying nitrogen conditions in the field and growth chamber. Ann Bot 2010, 105(7):1171-1182.
• [29]Becker H: Pflanzenzüchtung. Stuttgart: UTB; 2011. Volume 2
• [30]Dunnett CW: A multiple comparison procedure for comparing several treatments with a control. J Am Stat Assoc 1955, 50(272):1096-1121.
• [31]Huang YZ, Zhang GP, Wu FB, Chen JX, Xiao YP: Interaction of salinity and cadmium stresses on antioxidant enzymes, sodium, and cadmium accumulation in four barley genotypes. J Plant Nutr 2006, 29(12):2215-2225.
• [32]Bahrman N, Gouy A, Devienne-Barret F, Hirel B, Vedele F, Le Gouis J: Differential change in root protein patterns of two wheat varieties under high and low nitrogen nutrition levels. Plant Sci 2005, 168(1):81-87.
• [33]Le Bot J, Adamowicz S, Robin P: Modelling plant nutrition of horticultural crops: a review. Sci Hort 1998, 74(1–2):47-82.
• [34]Mattsson M, Johansson E, Lundborg T, Larsson M, Larsson CM: Nitrogen-utilization in N-limited barley during vegetative and generative growth .1. Growth and nitrate uptake kinetics in vegetative cultures grown at different relative addition rates of nitrate-N. J Exp Bot 1991, 42(235):197-205.
• [35]Ellis RP, Forster BP, Gordon DC, Handley LL, Keith RP, Lawrence P, Meyer R, Powell W, Robinson D, Scrimgeour CM, et al.: Phenotype/genotype associations for yield and salt tolerance in a barley mapping population segregating for two dwarfing genes. J Exp Bot 2002, 53(371):1163-1176.
• [36]Jana S, Wilen RW: Breeding for abiotic stress tolerance in barley. In Abiotic stress: plant resistance through breeding and molecular approaches. Edited by Ashraf M, Harris PJC. Binghampton, NY: CRC; 2005:491-511.
• [37]Ceccarelli S: Adaptation to low/high input cultivation. Euphytica 1996, 92(1–2):203-214.
• [38]Andersen S: Relation between leaf number and ear development in spring-sown barley and oats. Physiol Plantarum 1955, 8:404-417.
• [39]Dunbabin V, Diggle A, Rengel Z: Is there an optimal root architecture for nitrate capture in leaching environments? Plant Cell Environ 2003, 26(6):835-844.
• [40]Hansson AC, Pettersson R, Paustian K: Shoot and root production and nitrogen uptake in barley, with and without nitrogen fertilization. J Agron Crop Sci 1987, 158(3):163-171.
• [41]Chapin FS, Walter HS, Clarkson DT: Growth response of barley and tomato to nitrogen stress and its control by abscisic acid, water relations and photosythesis. Planta 1988, 173:352-366.
• [42]Cartelat A, Cerovic ZG, Goulas Y, Meyer S, Lelarge C, Prioul JL, Barbottin A, Jeuffroy MH, Gate P, Agati G, et al.: Optically assessed contents of leaf polyphenolics and chlorophyll as indicators of nitrogen deficiency in wheat (Triticum aestivum L.). Field Crop Res 2005, 91(1):35-49.
• [43]von Korff M, Wang H, Leon J, Pillen K: AB-QTL analysis in spring barley. I. Detection of resistance genes against powdery mildew, leaf rust and scald introgressed from wild barley. Theor Appl Genet 2005, 111(3):583-590.
• [44]von Korff M, L on J, Pillen K: Detection of epistatic interactions between exotic alleles introgressed from wild barley (Hordeum vulgare ssp. spontaneum). Theor Appl Genet 2010, 121(8):1455-1464.
• [45]von Korff M, Wang H, L on J, Pillen K: AB-QTL analysis in spring barley: II. Detection of favourable exotic alleles for agronomic traits introgressed from wild barley (H. vulgare ssp. spontaneum). Theor Appl Genet 2006, 112(7):1221-1231.
• [46]Cuesta-Marcos A, Casas AM, Yahiaoui S, Gracia MP, Lasa JM, Igartua E: Joint analysis for heading date QTL in small interconnected barley populations. Mol Breed 2008, 21(3):383-399.
• [47]Chen A, Baumann U, Fincher GB, Collins NC: Flt-2L, a locus in barley controlling flowering time, spike density, and plant height. Funct Integr Genomic 2009, 9(2):243-254.
• [48]Yan L, Fu D, Li C, Blechl A, Tranquilli G, Bonafede M, Sanchez A, Valarik M, Yasuda S, Dubcovsky J: The wheat and barley vernalization gene VRN3 is an orthologue of FT. Proc Natl Acad Sci USA 2006, 103(51):19581-19586.
• [49]Laurie DA, Pratchett N, Bezant JH, Snape JW: RFLP mapping of 5 major genes and 8 quantitative trait loci controlling flowering time in a winter x spring barley (Hordeum vulgare L.) cross. Genome 1995, 38(3):575-585.
• [50]Distelfeld A, Li C, Dubcovsky J: Regulation of flowering in temperate cereals. Curr Opin Plant Biol 2009, 12(2):178-184.
• [51]Dahleen LS, Vander Wal LJ, Franckowiak JD: Characterization and molecular mapping of genes determining semidwarfism in barley. J Hered 2005, 96(6):654-662.
• [52]Wang SY, Ward RW, Ritchie JT, Fischer RA, Schulthess U: Vernalization in wheat II. Genetic variability for the interchangeability of plant age and vernalization duration. Field Crop Res 1995, 44(2–3):67-72.
• [53]Gregory PJ, Bengough AG, Grinev D, Schmidt S, Thomas WTB, Wojciechowski T, Young IM: Root phenomics of crops: opportunities and challenges. Funct Plant Biol 2009, 36(10–11):922-929.
• [54]Laurie DA, Pratchett N, Romero C, Simpson E, Snape JW: Assignment of the denso dwarfing gene to the long arm of chromosome 3(3H) of barley by use of RFLP Markers. Plant Breed 1993, 111(3):198-203.
• [55]Hellewell KB, Rasmusson DC, Gallo-Meagher M: Enhancing yield of semidwarf barley. Crop Sci 2000, 40(2):352-358.
• [56]Chloupek O, Forster BP, Thomas WTB: The effect of semi-dwarf genes on root system size in field-grown barley. Theor Appl Genet 2006, 112(5):779-786.
• [57]Li M, Kudrna D, Kleinhofs A: Fine mapping of a semi-dwarf gene brachytic1 in barley. Barley Genet Newsl 2000, 32:14-17.
• [58]Faure S, Higgins J, Turner A, Laurie DA: The FLOWERING LOCUS T-like gene family in barley (Hordeum vulgare). Genetics 2007, 176(1):599-609.
• [59]Wojciechowski T, Gooding MJ, Ramsay L, Gregory PJ: The effects of dwarfing genes on seedling root growth of wheat. J Exp Bot 2009, 60(9):2565-2573.
• [60]Wang Y, Li H, Zhang L, Lü W, Wang J: On the use of mathematically-derived traits in QTL mapping. Mol Breed 2012, 29(3):661-673.
• [61]Babb S, Muehlbauer GJ: Genetic and morphological characterization of the barley uniculm2 (cul2) mutant. Theor Appl Genet 2003, 106(5):846-857.